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《Chinese Traditional and Herbal Drugs》 2013-23
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Subcellular localization of a sesquiterpene synthase from Aquilaria sinensis utilizing three transient expression systems

ZHAO Wen-ting;WEI Jian-he;MENG Dong;LIU Xiao-dong;GAO Zhi-hui;Institute of Medicinal Plant Development,Chinese Academy of Medical Science & Peking Union Medical College;Northeast Forestry University;Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine,Hainan Branch,Institute of Medicinal Plant Development Chinese Academy of Medical Science & Peking Union Medical College;China Agricultural University;  
Objective To investigate the subcellular localization of a sesquiterpene synthase from Aquilaria sinensis(ASS) and compare three transient expression systems in the subcellular localization study.Methods An ASS gene was amplified by RT-PCR using specific primers and cloned into the pEZS-NL and pFGC5941GFP to generate two plant expression vectors:pEZS-NL-ASSGFP and p5941-GFP-ASS.Three transient expression systems,agroinfiltration of tobacco leaves,particle bombardment of onion epidermal cells,and PEG transformation of protoplasts isolated from ASS calli were adopted and compared.The expression of the GFP fusion proteins were observed by a confocal laser scanning microscopy.Results The green fluorescence could be observed in all the three systems.However,the results were comparatively different.The cytoplasm and plastid localization of the GFP fusion protein observed in protoplasts was comparatively clear and was consistent with the studies in other plant species.Conclusion The results demonstrate that the ASS is located in the cytoplasm and plastid in the ASS protoplasts and the different locations in three systems might be caused by the distinct characters of the heterologous or homologous cells.
【Fund】: 国家自然科学基金资助项目(81001607 81173481);; 教育部新世纪优秀人才支持计划项目(2008);; 协和学者特聘教授计划(医科人发[2012]282号)
【CateGory Index】: S567.19;R282
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【References】
Chinese Journal Full-text Database 1 Hits
1 ZHAO Wen-Ting;GAO Zhi-Hui;MENG Dong;LIU Xiao-Dong;WEI Jian-He;Institute of Medicinal Plant Development,Chinese Academy of Medical Science & Peking Union Medical College;Northeast Forestry University;China Agricultural University;Hainan Branch,Institute of Medicinal Plant Development(Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine),Chinese Academy of Medical Sciences & Peking Union Medical College;;Construction of the in vivo Validation Systems of Plant microRNA Functions[J];生物技术通讯;2014-01
【Co-references】
Chinese Journal Full-text Database 5 Hits
1 ZHAO Wen-ting;WEI Jian-he;MENG Dong;LIU Xiao-dong;GAO Zhi-hui;Institute of Medicinal Plant Development,Chinese Academy of Medical Science & Peking Union Medical College;Northeast Forestry University;Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine,Hainan Branch,Institute of Medicinal Plant Development Chinese Academy of Medical Science & Peking Union Medical College;China Agricultural University;;Subcellular localization of a sesquiterpene synthase from Aquilaria sinensis utilizing three transient expression systems[J];中草药;2013-23
2 ZHAO Wen-Ting1,2,WEI Jian-He2,LIU Xiao-Dong1,GAO Zhi-Hui2 1.College of Landscape Architecture,Northeast Forestry University,Harbin 150040;2.Institute of Medicinal Plant Development,Chinese Academy of Medical Science,Malianwabei Road,Beijing 100193;China;Advance of the Main Methods and Applications of Plant Transient Expression System[J];生物技术通讯;2013-02
3 XIONG Wei~1,DONG Shaohong~1,YUAN Jianhui~2,LI Jianghua~1,LIU Jianjun~2,XU Xinyun~2 1 Department of Cardiology,Second Clinical Medical College of Jinan University,Shenzhen People's Hospital;2 Key Laboratory of Modern Toxicology in Shenzhen/Shenzhen Center for Disease Control and Prevention,Shenzhen 518020,China;MiRNA-146a promotes proliferation and migration of rat vascular smooth muscle cells in vitro in a nuclear factor-KB-dependent manner[J];南方医科大学学报;2012-02
4 Zeng Changying,Chen Xin,Lu Cheng,Wang Wenquan,Peng Ming Institute of Tropical Bioscience and Biotechnology,CATAS,Haikou,Hainan 571101,China;Plant Stress Resistance-related miRNA and Its Application in Cassava[J];热带作物学报;2010-02
5 WANG Lei, FAN Yun-liu (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, Beijing 100081, China);Progress of MicroRNA in Plants[J];中国农业科技导报;2007-03
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 Zhang Dayan;Jia Ling;Xiang Zhonghuai;He Ningjia;State Key Laboratory of Silkworm Genome Biology,Institute of Sericulture and Systems Biology,Southwest University;;Construction of Mulberry Transient Expression System Mediated by Agrobacterium and Expression Analyses of miRn51 and Its Target Gene[J];蚕业科学;2015-01
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