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《Chinese Traditional and Herbal Drugs》 2013-23
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HPLC fingerprint and chemical pattern recognition of water soluble components from Evodiae Fructus

WEI Juan;ZENG Jin-xiang;BI Ying;ZHANG Shou-wen;ZHANG Zhong-li;WU Bo;WEI Guo-bing;Germplasm Resource Engineering Technology Center of Jiangxi Province,Jiangxi College of Traditional Chinese Medicine;  
Objective To establish a method for HPLC fingerprint and chemical pattern recognition of the water soluble component from Evodiae Fructus,so as to provide the reference for the quality evaluation of Evodiae Fructus.Methods The chromatographic separation was performed on a C18 column(150 mm × 4.6 mm,3 μm).The mobile phase was a mixture of acetonitrile-0.5% acetic acid and 0.04% phosphoric acid aqueous solution in gradient elution.The flow rate was 0.8 mL/min,the detection wavelength was 327 nm,the injection volume was 10 μL,and the column temperature was 25 ℃.Results The HPLC fingerprint of 34 batches of Evodiae Fructus was set up and 27 common peaks were obtained.The similarity was between 0.945 and 0.997.The three species of Evodiae Fructus in Chinese Pharmacopeia 2010 could be completely discriminated by cluster analysis for the first time.The relationship among the different species of Evodiae Fructus were investigated from the water soluble components.The results of the principal component analysis were similar with the cluster analysis.Conclusion The cluster analysis method could be used for the quality control and comprehensive evaluation of Evodiae Fructus.
【Fund】: 国家“十二五”科技支撑计划资助项目(2011BAI04B04);; 江西中医药大学校级中医药课题(ZX1013)
【CateGory Index】: R284
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