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《Chinese Traditional and Herbal Drugs》 2014-01
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Comparative study on in vivo pharmacokinetics of simulated combination of berberine and other ingredients in Huanglian Jiedu Decoction in rats with cerebral ischemia

WANG Wen-zhe;ZHOU Jing;GUO Li-wei;ZHU Hua-xu;PAN Lin-mei;PAN Yong-lan;Key Laboratory of Separation Engineering of Chinese Materia Medica Compound,College of Pharmacy,Nanjing University of Chinese Medicine;  
Objective To compare the pharmacokinetic changing process of the main component berberine in four different simulated combinations based on the proportion of Huanglian Jiedu Decoction(HJD) in rats with cerebral ischemia. Methods According to body weight the rats were divided into model and Sham groups, and the middle cerebral artery occlusion(MCAO) was reproduced by suture method to make rats with cerebral ischemia in the model group. Each group was divided into four sub-groups: berberine(247.4 mg/kg), berberine(247.4 mg/kg) + baicalin(190.7 mg/kg), berberine(247.4 mg/kg) + geniposide(149.5 mg/kg), and berberine(247.4 mg/kg) + geniposide(149.5 mg/kg) + baicalin(190.7 mg/kg) groups. After ig administration, orbital blood was collected in 0.083, 0.25, 0.5, 1, 2, 3, 4, 6, 8, 10, 12, 24, 48, and 72 h, respectively. HPLC method was used for the determination of berberine concentration change in rat plasma. The concentration-time curve was plotted and the pharmacokinetic parameters were calculated by DAS 2.0. Results According to the statistical parameters, the absorbed order of berberine in the model and Sham groups was as follows: berberine + geniposide berberine + geniposide + baicalin berberine berberine + baicalin. Conclusion For MCAO rats, geniposide in HJD could promote the absorption of berberine, and baicalin could inhibit the absorption of berberine, but the combination of the three could alleviate the inhibition of berberine absorption by baicalin.
【Fund】: 江苏省自然科学基金资助项目(BK2012855);; 江苏省中医药管理局资助项目(LZ11199);; 江苏省高校优势学科项目;; 南京中医药大学中医学优势学科开放课题(S2012ZYX310)
【CateGory Index】: R285.5
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