Authentication of DNA barcoding of Scutellaria baicalensis and its related species
XIA Zhi;Feng Cui-yuan;GAO Zhi-ming;LI He-min;ZHANG Hong-rui;Engineering Technology Research Center for Chinese Medicine of Agronomy College,Henan Agricultural University;
Objective To fastly and acurrately authenticate Scutellaria baicalensis and its closely related species using DNA barcoding technique. Methods Total genomic DNA was isolated from S. baicalensis and its related species. Nuclear DNA ITS and chloroplast gene psbA-trnH sequences were amplified and sequenced. The Kimura 2-parameter(K2P) distances were calculated. Identification analyses were performed using BLAST1, nearest distance, and Neighbor-joining(NJ) methods. Results The genetic distances ofITS and psbA-trnH between S. baicalensis and its closely related species were(0.067 97—0.088 80) and(0.050 61—0.057 37), which were obviously higher than those in the intra-species of S. baicalensis(0—0.006 40) and(0—0.003 11). The NJ tree of ITS and psbA-trnH indicated that the eight geographical populations of S. baicalensis formed a monophyletic clade [bootstrap(BS) = 100%]. All the three methods showed that ITS and psbA-trnH could discriminate S. baicalensis from its closely related species correctly. Conclusion ITS and psbA-trnH are two efficient barcodes for the authentication of S. baicalensis and its related species.