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《Chinese Traditional and Herbal Drugs》 2015-01
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Proteomics research on antifibrotic mechanism of combination therapy based on isobaric tags for relative and absolute quantitation technique

CAO Wen;LIAO Ming;ZHOU Yan;Department of Pharmacology, Guangxi Medical University;Key Laboratory of High-Incidence-Tumor Prevention & Treatment, Medical Scientific Research Centre, Guangxi Medical University;  
Objective To investigate the protein basis and molecular mechanism of combination therapy with epigallocatechin gallate(EGCG), taurine, and genistein on CCl4-induced liver fibrosis in rats using isobaric tags for relative and absolute quantitation(i TRAQ). Methods The SD rats were randomly divided into normal control, model, and combination therapy(taurine 200 mg/kg + EGCG 30 mg/kg + genistein 20 mg/kg) groups. The rats in the model and combination therapy groups were induced by ig administration of 50% CCl4 for 14 weeks, and the rats in the normal control group were given peanut oil. The combination therapy was started at week 9 for 6 weeks. HE staining of liver tissue was performed to evaluate histopathologic changes. The high-abundance proteins in the serum of model and combination therapy groups were depleted by Proteo Miner Protein Enrichment Kit. Proteins differentially expressed in the serum of those two groups were identified with i TRAQ coupled with LC-ESI-MS/MS technology and analyzed with bioinformatics tools. The expression of representative differential protein(Txn1) was validated by ELISA. Results A total of 359 proteins with the confidence coefficient above 95% were identified, of which 78 were differential expressed proteins, including 51 up-regulated ones and 27 down-regulated ones. The serum level of Txn1 increased significantly in the combination therapy group compared with the model group(P 0.05). Conclusion The combination therapy can alleviate the progression of liver fibrosis effectively by affecting multiple biological processes. The regulation of anti-oxidant defense system and coagulation cascade pathway may be the molecular mechanisms of the combination therapy against liver fibrosis.
【Fund】: 国家自然科学基金资助项目(81160063 81360192);; 广西自然科学基金项目(2013GXNSFBA019167 2012GXNSFCA053004);; 广西区域性高发肿瘤早期防治研究重点实验室基金(GK2013-13-A-01-03)
【CateGory Index】: R285.5
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 Bai Xiao-su Ren Wei Zhang Su-hua Department of Endocrinology First Affiliated Hospital Chongqing University of Medical Sciences Chongqing 400016 China;The latest studies and present status of the application of serum proteomics[J];Chinese Journal of Clinical Rehabilitation;2005-27
2 Ming Liao, Xing Lin, Zhao-Ni Chen, Guangxi Medical University, Key Laboratory for Early Prevention and Treatment of Endemic Tumors, Ministry of Education, Nanning 530021, Guangxi Zhuang Autonomous Region, China Yan Li, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region, China Lang Zhuo, Institute of Bioengineering and Nanotechnology, Biopolis 169483, Singapore;Effects of cocktail therapy on TGF-β1,COL Ⅰ and COL Ⅲ expression in liver fibrosis in rats[J];World Chinese Journal of Digestology;2010-18
3 ZHOU Cheng-yan,AI Ling-yan,WANG Mei,WANG Xu Department of Pharmacy,Hebei Uinited University,Tangshan 063000,China;Protection of Eucommia ulmoides polysaccharide on liver fibrosis[J];Chinese Traditional and Herbal Drugs;2011-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 MA Bo;ZHANG Yuan;ZHANG Dayi;TONG Yu;XIAO Zhengquan;Beijing Beiya Orthopedics Hospital;Institute of Medicinal Plant Development in Chinese Academy of Medical Sciences and Peking Union Medical College;;Advance at Chemical Ingredients and Pharmacological Activity of DuZhong[J];Western Journal of Traditional Chinese Medicine;2013-12
2 WANG Kai;LU Xi;LIU Yan-fen;CHEN Shao-hong;LIU You;Agricultural College of Guangdong Ocean University;Biochemistry Center of Guangdong Ocean University;;Adjuvant Effects of Eucommia ulmoides Oliv.Polysaccharide on Immune Response of Sanhuang Chicken[J];Journal of Henan Agricultural Sciences;2014-01
3 ZHANG Jingjing;DU Hongyan;LI Qin;DING Yanxia;Eucommia ulmoides Cultication and Utilization of Henan Engineering Laboratory;pharmaceutical of College of Henan University;Eucommiaulmoides Cultication and Utilization of Henan Colleges and Universities Engineering Research Center;Non-timber forest Research and devlopment center of CAF;;Study advancement about pharmacological andtoxicological of Eucommia ulmoides Oliv[J];Journal of Henan University(Medical Science);2014-03
4 Cheng Yawei1,He Lei1,Liao Ping2,Hu Heng2,Jin Yaming3,Li Fufeng1,Wang Wenjing2,Hao Yiming1,Wang Yiqin1 (1.Laboratory of Syndrome of TCM,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;2.Department of Molecular Biology for Public Health,Shanghai Municipal Center for Disease Control and Prevention,Shanghai 200336,China;3.Nephrology of Longhua Hospital of Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China);Study on Proteinum in Clear Supernatant Liquid of Tongue Coating of Chronic Renal Failure Based on Protein Chip[J];World Science and Technology(Modernization of Traditional Chinese Medicine and Materia Medica);2011-04
5 Tan Qin-xiang Lü Zhi-ping Zhang Xu-fu Zhong Xiao-lan Department of Traditional Chinese Medicine Southern Medical University Guangzhou 510515 Guangdong Province China;Application of proteome in liver-qi stagnation syndrome[J];Chinese Journal of Clinical Rehabilitation;2006-11
6 Ming Liao, Yi Zhou, Min He, Guangxi Medical University, Guangxi Key Laboratory for Early Prevention & Treatment of Endemic Tumors, Ministry of Education, Nanning 530021, Guangxi Zhuang Autonomous Region, China Cai-Feng Mo, Guilin Medical University, Guilin 541004, Guangxi Zhuang Autonomous Region, China Lang Zhuo, Institute of Bioengineering and Nanotechnology, Singapore 169483;Alterations in fibrosis-related gene expression and proteomic expression profile in rat hepatic stellate cells exposed to the cocktail[J];World Chinese Journal of Digestology;2011-17
7 HE Changsheng1 LI Yuanyuan2 YUE Hongyi1 XUE Feng1 LIU Jian1 1.Department of Hepatobiliary Surgery,Jiaozhou Central Hospital of Qingdao,Shandong Province,Qingdao 266300,China;2.Department of Pediatrics,the First people′s Hospital of Baoding,Hebei Province,Baoding 071000,China;Protective effects of Artemisia capillaries polysaccharides for hepatic fibrosis on immature rats with obstructive jaundice[J];China Medical Herald;2012-08
8 PAN Zan-hong,MI Yu-qiang,BI Li-ping,WU Xi-liang Tianjin Hospital for Infectious Diseases,Tianjin 300192,China;Effect of Ruangan Huaxian Pills on hepatic fibrosis in rats induced by CCl_4[J];Chinese Traditional and Herbal Drugs;2011-05
9 YANG Yue-hui1,JIANG Qing-hua1,DING Ping-tian2 1.Shengjing Hospital Affiliated China Medical University,Shenyang 110004,China 2.Shenyang Pharmaceutical University,Shenyang 110016,China;Advances in studies on analytical methods of polysaccharides in plant[J];Chinese Traditional and Herbal Drugs;2011-06
10 NI Chun-yan,ZHENG Shi-zhong,LU Yin,ZHANG Xue-jiao,MA Jin,WANG Ai-yun Department of Clinical Pharmacy,College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210029,China;Strategy and progress on monomer components of Chinese materia medica for prevention and treatment of hepatic fibrosis with leptin as target[J];Chinese Traditional and Herbal Drugs;2011-09
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 FENG Yi-zhong1,ZHANG Yong-sheng2,4,CAO Zhi-fei2,GU Zhen-lun2,YANG Qing-yao3,YANG Xiao-tong3,CHOU Wen-hsien5,KWOK Chi-yi5 P1.Department of Pathology,Soochow University,Suzhou 215004,China 2.Suzhou Institute of Chinese Materia Medica,Suzhou 215007,China 3.Shanghai Yang's Herb Institute,Shanghai 200233,China 4.Department of Pathology,the Second Hospital Affiliated to Soochow University,Suzhou 215004,China 5.The Hong Kong Association for Health Care Ltd.,Hong Kong,China;Prevention of Bleomycin-induced Pulmonary Fibrosis in Mice by the Combination of Hirsutella sinensis and Panax notoginseng Extracts[J];中草药(英文版);2010-02
2 DENG Xin1,LIANG Jian1,HUANG Bin1,LI Yi-zhong1,QIN Li-kui1,ZHANG Xi-liu2(1.The Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College,Nanning 530011,China;2.The First Affiliated Hospital of Guangxi Traditional Chinese Medical College,Nanning 530011,China);Effects of natural taurine on TGF-β_1/Smad singaling pathway in rats of hepatic fibrosis[J];Journal of Dalian Medical University;2007-04
3 Yue-Xiang Chen, Xing-Rong Zhang, Wei-Fen Xie and Shi Li Shanghai, China Department of Gastroenterology, Changzheng Hospi- tal , Second Military Medical University, Shanghai 200003, China;Effects of taurine on proliferation and apoptosis of hepatic stellate cells in vitro[J];国际肝胆胰疾病杂志(英文版);2004-01
4 Wei Xin,Liang Jian,Zhang Xiliu,et al. Department of Digestion,The Frist Affiliated Hospital of Guangxi college of Traditional Chinese Medicine(Nanning 530023);Effects of taurine on the expression of transforming growth factor β_1 and tumor necrosis factor α in rats with liver fibrosis[J];Guangxi Medical Journal;2003-11
5 ZHOU Cheng-yan1,YU Hai-ping2,TAO Zhi-bin1,GAO Yan-shan1,BIAN Hong-rong1(1.North China Coal Medical College,Tangshan 063000,China;2.Tangshan Traditional Chinese Medicine Hospital,Tangshan 063000,China);Protective Effect of the Water Extract from Eucommia ulmoides Oliver against Liver Injury in Mice[J];Lishizhen Medicine and Materia Medica Research;2009-09
6 Guo-Qiang Bao, Su-Bo Li, Yang-Pei Zhang, Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, china Yong-Xiong Zhang, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China Shi-Wen Wang, Institute of Geriatric Cardiology, General Hospital of PLA, Beijing 100853, China;Difference of lungs protein expression between aged and young mice[J];Chinese Journal of Clinical Rehabilitation;2004-09
7 Ming Liao,Wei Shu,Medical Scientifi c Research Center of Guangxi Medical University,Key Laboratory for Early Prevention and Treatment of Endemic Tumors,Ministry of Education,Nanning 530021,Guangxi Zhuang Autonomous Region,China Yan Li,Guangxi University,Nanning 530004,Guangxi Zhuang Autonomous Region,China;Effects of genistein on cell proliferation and TGF-β1,MMP-2 and TIMP-2 expression in rat hepatic stellate cells[J];World Chinese Journal of Digestology;2010-03
8 WANG Gen-Sheng,HAN Zhe-Wu (Department of pharmacology, Inner Mongolia Medical College, Huhhot 010059);Effects of flavonoids of glycyrrhiza on ethanol-induced liver injury in mice[J];Chinese Pharmacological Bulletin;1993-04
9 ZHENG Qing-Shan, ZHANG Jing-Song, GUI Chang-Qing, WANG Xue-Wen, YANG Hui-Jie, LI Ai-Hua, WANG You-Xia, SUN Rui-Yuan (Anhui Provincial Center of Drug Clinical Evaluation, Yijishan Hospital of Wanan Medical College, Wuhu 241001);Quantitative analyses of the combined effects of nano red elemental selenium and glucurolactone on forming liver fibrosis induced by CCl_4 in rats[J];Chinese Pharmacological Bulletin;2002-01
10 ZENG You-xiang~(1),YANG Wei-feng~(2),LIU Ling-yun~(3)(1.Department of Internal Medicine,the Shaoyang Municipal Hospital of Traditional Chinese Medicine Integrated with Western Medicine,Shaoyang 421001,China;2.Department of Internal Disease of the Digestive System,Xiangya Second Hospital,Central South China University,Changsha 410013,China;3.Department of Pharmacy,the First Hospital Affiliated with the Hunan University of Traditional Chinese Medicine,Changsha 410013 China);An Experimental Study of the Prevention of Liver Fibrosis by Amiloride Combined with Oxymoron[J];Herald of Medicine;2006-10
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