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《Chinese Traditional and Herbal Drugs》 2016-02
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Fingerprint and multi-components quantitative determination for Huoxuetongluo Injection by UPLC

LIN Xia;CUI Pei-chao;XU Gui-hong;LI Jia-chun;HUANG Wen-zhe;WANG Zhen-zhong;XIAO Wei;College of Pharmacy, Nanjing University of Chinese Medicine;Jiangsu Kanion Pharmaceutical Co., Ltd.;State Key Laboratory of Pharmaceutical Process New-tech for Chinese Medicine;Jiangsu Kanion Ecological Agriculture Development Co., Ltd.;  
Objective To establish a UPLC fingerprint of the compounds in Huoxuetongluo Injection, and to make a quantitative analysis. Methods The Thermo C18(100 mm × 3 mm, 1.7 μm) column was used with a mobile phase of methyl alcohol-0.05% formic acid gradient elution, the flow rate was 0.4 m L/min, the column temperature was 20 ℃, and the detection wavelength was 260 nm. Results The fingerprint chromatography included 21 mutual peaks, of which 11 mutual peaks from Paeoniae Radix Rubra, 7 mutual peaks from Chuanxiong Rhizoma, and 3 mutual peaks from Persicae semen. The similarity among the batches was more than 0.98. Based on the retention time, and UV absorption spectra of reference compounds, five components, amygdalin, oxypaeoniflorin, albiflorin, paeoniflorin, and ferulic acid, were identified and quantified. Conclusion The method is rapid, simple, and accurate, and can be used for the quality control of Huoxuetongluo Injection.
【Fund】: 国家科技部重大新药创制“脑血栓形成急性期治疗药物——注射用活血通络的临床研究”(2011ZX091012-122)
【CateGory Index】: R286.0;O657.7
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