Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Traditional and Herbal Drugs》 2016-02
Add to Favorite Get Latest Update

Cloning and expression analysis of WRKY5 gene in Dendrobium officinale

JIANG Yuan;ZHU Yu-qiu;GAO Yan-hui;SI Jin-ping;Nurturing Station for State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Zhejiang Provincial Strategic Alliance for Technical Innovation in Industry of Dendrobium officinale;  
Objective In order to isolate and analysize the bioinformatics and expression pattern of Do WRKY5 gene from Dendrobium officinale. Methods A WRKY gene was first obtained by transcriptome sequencing and reverse transcription-polymerase chain reaction(RT-PCR) from D. officinale and analyzed by bioinformatics tools. The tissue expression pattern and the low temperature stress, abscisic acid(ABA) stress, and sucrose stress responses were analyzed by q RT-PCR. Results The c DNA sequence of Do WRKY5 gene was isolated, which was 1 336 bp in length, with an open reading frame(ORF) of 834 bp and an encoded polypeptide of 277 amino acid. The amino acid sequence contained a conserved WRKY domains and a zinc finger structures(C2H2), belonging to GroupⅡof WRKY family. Expression analysis by q RT-PCR showed that Do WRKY5 was expressed in the roots, stems, and leaves of D. officinale, and the most abundant in leaves. The amount of Do WRKY5 expression were significantly increased under low temperature of 4 ℃ and different time. Moreover, the expression of Do WRKY5 could be induced by ABA and source. Conclusion Do WRKY5 may be an important transcription factor to response cold stress and other abiotic stresses in D. officinale, which provides a foundation for further study of cold tolerance mechanism and cold-resistant breeding of D. officinale.
【Fund】: 浙江省重大科技专项(2012C12912-9);; 浙江省林学重中之重一级学科研究生创新项目(201530)
【CateGory Index】: S567.239
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved