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Analysis on chemical constituents of Pheretima aspergillum by UPLC-Q-TOF-MS

ZHANG Yu;DONG Wen-ting;HUO Jin-hai;WANG Wei-ming;Heilongjiang Academy of Chinese Medical Sciences;  
Objective To establish a method for the analysis of constituents of Pheretima aspergillum by UPLC-Q-TOF-MS. Methods The analysis was performed on an Acquity UPLC BEH C_(18) column(100 mm× 2.1 mm, 1.7 μm) by gradient elution. The mobile phase consist of 0.1% formic acid-acetonitrile and 0.1% formic acid-water at a flow rate of 0.3 m L/min. The column temperature was at 35 ℃. The information of the compounds was acquired in positive and negative mode. Results Total 83 compounds were inferred, contains 11 free amino acids, 26 organic acids, 11 necleosides, 5 dipeptides and cyclic dipeptideds and other 21 nitrogen-containing substances, the rest on a total of 10. Conclusion The method is accurate, rapid, and sensitive, and provide a basis for further clarifying the material basis of its efficacy and the selection of quality control indicators.
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