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Research on biotransformation of saikosaponin A in vitro based on HPLC-DAD-MS~n

YU Bei-bei;WANG Liang;YIN Li-shun;SUN Rong;Shandong Academy of Chinese Medicine;Key Discipline of Traditional Chinese Medicine Chemistry of Shandong Administration of Traditional Chinese Medicine;Shandong University of Traditional Chinese Medicine;Key Discipline of Traditional Chinese Medicine Pharmacology of Shandong Administration of Traditional Chinese Medicine;  
Objective To study the biotransformation of saikosaponin A in vitro and analyze its metabolites. Methods Saikosaponin A was incubated in artificial gastric juice and intestinal contents of rats in anaerobic conditions, respectively, and the metabolites were analyzed and identified by HPLC-DAD-MS~n. Results By incubation of saikosaponin A in artificial gastric juice, saikosaponin b1 and g were detected in the reaction mixture. By the anaerobic incubation of saikosaponin A with intestinal flora, prosaikogenin F was soon detected, but it was further converted to saikogenin F. Conclusion Saikosaponin A can be transformed to secondary glycosides and glycosides in artificial gastrointestinal environment. And the products can be identified by HPLC-DAD-MS~n. The distribution and concentration of saikosaponin A in vivo can not reflect its fate fully, and the metabolites should be considered simutaneously.
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