Analysis of genetic diversity and construction of fingerprint on Tamarix chinensis from Qinhuangdao by ISSR
YANG Qing;YANG Fei;YANG Kang;CAO Shu-min;FANG Cong-cong;JIA Xue-ying;YANG Jun-ming;College of Life Sciences and Technology,Hebei Normal University of Science & Technology;College of Horticulture Science and Technology,Hebei Normal University of Science & Technology;
Objective To study the genetic diversity and phylogenetic relationship on Tamarix chinensis of Qinhuangdao in Hebei province, and to establish their DNA fingerprints, which will provide some guidance for crossbreeding and cultivar identification of T. chinensis. Methods Inter-simple sequence repeat(ISSR) was conducted to assess the genetic diversity of 32 T. chinensis individuals and the DNA fingerprints were established by Popgene software. Results Nine ISSR primers produced 114 loci, of which 110 were polymorphisms, and the percentage of polymorphic loci was up to 96.49% among 32 individuals. The average value of the effective number of alleles, Nei?s genetic diversity and Shannon?s information index were 1.543 7, 0.320 3, and 0.484 2, respectively. Based on the unweighted pari group method with arithmetic average(UPGMA) and ISSR molecular markers, the 32 T. chinensis were divided into two groups at the genetic distance of 28.79. In addition, the DNA fingerprints for 32 individuals were established by polymorphic ISSR primers. There existed abundant genetic diversity among 32 individuals. ISSR molecular markers could be effectively used in genetic diversity and fingerprint analysis for T. chinensis.