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《浙江大学学报(英文版)》 2005-01
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Science Letters:Serum protein fingerprinting coupled with artificial neural network distinguishes glioma from healthy population or brain benign tumor

LIU Jian~1 ZHENG Shu~1 YU Jie-kai(ZHANG Jian-min(张建民)~2 CHEN Zhe(陈喆)~1~1Cancer Institute~2Neurosurgery Department,Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310009,China  
To screen and evaluate protein biomarkers for the detection of gliomas(Astrocytoma grade Ⅰ-Ⅳ) from healthy in-dividuals and gliomas from brain benign tumors by using surface enhanced laser desorption/ionization time of flight mass spec-trometry(SELDI-TOF-MS)coupled with an artificial neural network(ANN)algorithm.SELDI-TOF-MS protein fingerprinting ofserum from 105 brain tumor patients and healthy individuals,included 28 patients with glioma(Astrocytoma Ⅰ-Ⅳ),37 patientswith brain benign tumor,and 40 age-matched healthy individuals.Two thirds of the total samples of every compared pair as trainingset were used to set up discriminating patterns,and one third of total samples of every compared pair as test set were used tocross-validate;simultaneously,discriminate-cluster analysis derived SPSS 10.0 software was used to compare Astrocytoma gradeⅠ-Ⅱ with grade Ⅲ-Ⅳ ones.An accuracy of 95.7%,sensitivity of 88.9%,specificity of 100%,positive predictive value of 90% andnegative predictive value of 100% were obtained in a blinded test set comparing gliomas patients with healthy individuals;anaccuracy of 86.4%,sensitivity of 88.9%,specificity of 84.6%,positive predictive value of 90% and negative predictive value of85.7% were obtained when patient's gliomas was compared with benign brain tumor.Total accuracy of 85.7%,accuracy of gradeⅠ-Ⅱ Astrocytoma was 86.7%,accuracy of Ⅲ-Ⅳ Astrocytoma was 84.6% were obtained when grade Ⅰ-Ⅱ Astrocytoma was comparedwith grade Ⅲ-Ⅳ ones(discriminant analysis).SELDI-TOF-MS combined with bioinformatics tools,could greatly facilitate thediscovery of better biomarkers.The high sensitivity and specificity achieved by the use of selected biomarkers showed greatpotential application for the discrimination of gliomas patients from healthy individuals and glioma from brain benign tumors.
【Fund】: Project(No.G1998051200)supportedbytheNationalBasicResearchProgram(973)ofChina
【CateGory Index】: R739.4
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【References】
Chinese Journal Full-text Database 3 Hits
1 WU Yu-mei,LI Xin-zheng,WANG Chun-yan,WANG Fu-hua,YANG Rui-hong,ZHANG Yun-peng,GUO Su-tang.College of Postgraduate,Shanxi Medical University,Taiyuan 030001,China;The applied research of SELDI proteomic patterns in diagnosis of breast cancer lymph node metastases[J];中华临床医师杂志(电子版);2012-12
2 ZHU Yang-wen1,WANG Yue-dong1,YE Zai-yuan1,HU Xun2,Yu Jie-kai2(1.Department of General Surgery,Zhejiang Provincial People's Hospital,Hangzhou 310014,China;2.Cancer Institute,Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310009,China);Application of serum protein fingerprint in diagnosis of pancreatic cancer[J];浙江大学学报(医学版);2012-03
3 YANG Rui-hong,HU Cheng-guang,LI Jie,WANG Jing,GUO Su-tang.Department of Molecular Biology,Shanxi Province Tumor Hospital and Research Institute,Taiyuan 030013,China;Application of surface-enhanced laser desorption/ionization-time of flight-mass spectrometry in early diagnosis and monitoring therapy of esophageal carcinoma[J];中华临床医师杂志(电子版);2011-15
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 PANG Da1, YANG Yanmei2, ZHANG Guoqiang3, MA Yuyan2, TANG Yali2, GONG Jianping11Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China 2Institute of Cancer Prevention and Treatment, Harbin Medical University, Harbin 150040, China 3The Tumor Hospital of Harbin Medical University, Harbin 150040, China;The Applied Research of Serum Protein Fingerprints in the Prediction of Axillary Lymph Nodes (ALN) Metastasis[J];中国肿瘤临床;2008-17
2 LIU Cha-Zhen1, ZHU Pei-Yun1, SHI Min-Xin2, LIU Ji-Bin3, LIAO Ping1, XIANG Cui-Qin1, ZHANG Yi-Xin2, WANG Wen-Jing11. Department of Molecular Biology for Public Health, Shanghai Municipal Center for Disease Control and Prevention, Shanghai, 200336, P. R. China2. Department of Oncologic Surgery, Nantong Tumor Hospital, Nantong, Jiangsu, 226361, P. R. China3. Cancer Institute, Nantong Tumor Hospital, Nantong, Jiangsu, 226361, P. R. China;Serum Proteomic Spectra of Esophageal Squamous Cell Carcinoma Patients Analyzed with IMAC3 Protein Chip[J];癌症;2008-03
3 FENG Xiao-shan1,2, WANG Li-dong1, GUO Tao1, SHAN Tan-you2, LI Ji-lin3, FAN Zong-min1, JIAO Xin-ying3, CHANG Zhi-hui4, HAN Jing2, SONG Xin1, GAO She-gan1,2, SHEN Qiu1, FAN Hui1, WANG Neng-chao1, LI Shao-hua1, GAO Shan-shan1, HE Xin1, GUO Jun-hui11. Henan Key Laboratory for Esophageal Cancer, Basic Medical College, Zhengzhou University, Zhengzhou 450052, P. R. China2. Department of Oncology, First Affiliated Hospital of Henan University of Science and Technology; Cancer Institute, Henan University of Science and Technology, Luoyang 471003, P. R. China3. Depertment of Pathology, Yaocun Esophageal Cancer Hospital, Linzhou 456592, P. R. China4. Depertment of Pathology, Linzhou Center Hospital, Linzhou 456550, P. R. China;Proteomic model for diagnosis and the detection of tumor markers in concurrent cancer of the esophagus and gastric cardia from the same patient[J];中华肿瘤防治杂志;2007-08
4 $ZHANG Li-wei1, WEN Deng-gui2, LI Ying-sai1, YU Wei-fang1 WANG Shun-ping1,ER Li-mian1,CONG Qing-wen1,WANG Jun-he3 LI Su-ping3, LI Yong-wei3, MA Cai-fen(3 SHAN Bao-en2, WANG Shi-jie1* 1 Department of Endoscopy, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011; 2 Hebei Cancer Institute, Shijiazhuang 050011;3Shexian County Cancer Institute, Shexian County 056400;EPIDEMIC STRENGTH OF CARDIA AND DISTANT STOMACH CANCER IN THE HIGH RISK REGION OF ESOPHAGEAL CANCER AND THEIR IMPLICATION TO EDOSCOPIC SCREENING[J];Chinese Journal of Cancer Research;2006-02
5 Judith YMN Engwegen, Jos H Beijnen, Department of Pharmacy and Pharmacology, The Netherlands Cancer Institute/ Slotervaart Hospital, Amsterdam, The Netherlands Helgi H Helgason, Jan HM Schellens, Department of Medical Oncology, The Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital, Amsterdam, The Netherlands Annemieke Cats, Department of Gastroenterology and Hepatology, The Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital, Amsterdam, The Netherlands Nathan Harris, Ciphergen Biosystems Inc., Freemont, California, United States Johannes MG Bonfrer, Department of Clinical Chemistry, The Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital, Amsterdam, The Netherlands Jan HM Schellens, Jos H Beijnen, Utrecht University, Faculty of Pharmaceutical Sciences, Department of Biomedical analysis, Utrecht, The Netherlands;Identification of serum proteins discriminating colorectal cancer patients and healthy controls using surface-enhanced laser desorption ionisation-time of flight mass spectrometry[J];World Journal of Gastroenterology;2006-10
6 WANG Jia-xiang, ZHANG Bo, YU Jie-kai, LIU Jian, YANG Mei-qin and ZHENG Shu Department of Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China Institute of Oncology, Zhejiang University, Hangzhou 310000, China;Application of serum protein fingerprinting coupled with artificial neural network model in diagnosis of hepatocellular carcinoma[J];Chinese Medical Journal;2005-15
7 YU Jie-kai ZHENG Shu TANG Yong , LI Li (1Cancer Institute, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China)(2School of Life Science, Zhejiang University, Hangzhou 310029, China) (3Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China);An integrated approach utilizing proteomics and bioinformatics to detect ovarian cancer[J];Journal of Zhejiang University Science;2005-04
8 LIU Jian~1 ZHENG Shu~1 YU Jie-kai(ZHANG Jian-min(张建民)~2 CHEN Zhe(陈喆)~1~1Cancer Institute~2Neurosurgery Department,Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310009,China;Science Letters:Serum protein fingerprinting coupled with artificial neural network distinguishes glioma from healthy population or brain benign tumor[J];Journal of Zhejiang University Science;2005-01
9 Zheng Ying, Li Delu, Xiang Yongmei, et al. Shanghai Municipal Center for Disease Control and Prevention, Shanghai(200336);The Status and Trend of Breast Cancer Incidence in Shanghai.[J];外科理论与实践;2001-04
10 Qiao You- lin# Hou Jun* Yang Ling He Yu- tong* Liu Yun- yuan Li Lian- di** Li Shao- sen*** Lian Shi- yong**** Dong Zhi- wei ( Department of Cancer Epidemiology, Cancer Institute, CAMS and PUMC, Beijing 100021 );The Trends and Preventive Strategies of Esophageal Cancer in High- risk Areas of Taihang Mountains, China[J];中国医学科学院学报;2001-01
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