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《浙江大学学报(英文版)》 2005-01
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Science Letters:Construction of a eukaryotic expression plasmid of Humanin

LUO Ben-yan~1 CHEN Xiang-ming TANG Min CHEN Feng(陈峰)~3 CHEN Zhi(陈智)~3~1Department of Neurology,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310003,China~2Department of Anesthesiology,Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310009,China~3Institute of Infectious Diseases,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310003.China  
Objective:To construct a eukaryotic expression plasmid pcDNA3.1(-)-Humanin.Methods:The recombinant plasmidpGEMEX-1-Humanin was digested with restriction endonucleases BamH Ⅰ and Hind Ⅲ and the Humanin gene fragments,about100 bp length,were obtained.Then the Humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1(-)andthe recombinant plasmids pcDNA3.1(-)-Humanin were identified by sequencing.Results:Recombinant plasmid DNA success-fully produced a band which had the same size as that of the thimauin positive control.The sequence of recombinant plasmidsaccorded with the Humnain gene sequence.Conclusions:A eukaryotic expression plasmid of Humanin was successfully con-structed.
【Fund】: Project(No.2003C30040) supportedbytheScienceandTechnologyDepartmentofZhejiangProvince China
【CateGory Index】: R346
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