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《浙江大学学报(英文版)》 2005-01
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The binding of MBL to common bacteria in infectious diseases of children

SHANG Shi-qiang~1 CHEN Guo-xian~2 SHEN Jie YU Xiao-hong WANG Ke-yi~1Affiliated Children's Hospital,Zhejiang University,Hangzhou 310003.China~2Second Affiliated Hospital,Zhejiang University,Hangzhou 310009.China~3Department of Biochemistry Zhejiang University,Hangzhou 310006.China  
Objective:To purify Mannan-binding lectin(MBL)from human serum and detect its binding ability to several kindsof bacteria common in infections diseases of children.Methods:MBL was purifide from human serum by affinity chromatographyon mannan-Sepharose 4B column.Its binding ability to eight species,97 stratus of bacteria was detected by enzyme-linked lectinassay(ELLA).Results:MBL has different binding ability to bacteria and shows strong binding ability to Klebsiella ornithinolvticaand Escherichia coli,but shows relatively lower binding ability to Staphylococcus haemolyticus,Enterobacter cloacae andStaphylococcus epidermidis.To different isolates of Klebsiella pneumoniae,Haemophilus influenzae and Staphylococcus aureus,MBL shows quite different binding ability.Conclusions:MBL has different binding ability to different bacteria,and has relativelystronger binding ability to Gram-negative bacteria.Its binding ability to different isolates of certain kinds of bacteria is quitedifferent.
【Fund】: Project(No R02061) supportedbyNaturalScienceFoundationofZhejiangProvince China
【CateGory Index】: R725.1
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1 WANG Min,HUA Chun-zhen,SHANG Shi-qiang,YU Hui-min(Department of Pediatrics,Clinical Medical School of Hangzhou Normal University,Hangzhou 310018,China);The single nucleotide polymorphism at-221 site in promoter region and at +230 site in exon I regions of mannose binding lectin gene from Hans children and its haplotype analysis[J];Current Immunology;2008-02
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