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《浙江大学学报B(生物医学、生物工程版)(英文版)》 2008-01
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Optimization of acidic extraction of astaxanthin from Phaffia rhodozyma

Hui NI1,2,Qi-he CHEN1,Guo-qing HE1,Guang-bin WU2,Yuan-fan YANG2 (1School of Biosystem Engineering and Food Science,Zhejiang University,Hangzhou 310029,China) (2School of Bioengineering,Jimei University,Xiamen 361021,China)  
Optimization of a process for extracting astaxanthin from Phaffia rhodozyma by acidic method was investigated,regarding several extraction factors such as acids,organic solvents,temperature and time. Fractional factorial design,central composite design and response surface methodology were used to derive a statistically optimal model,which corresponded to the following optimal condition: concentration of lactic acid at 5.55 mol/L,ratio of ethanol to yeast dry weight at 20.25 ml/g,tem-perature for cell-disruption at 30 °C,and extraction time for 3 min. Under this condition,astaxanthin and the total carotenoids could be extracted in amounts of 1294.7 μg/g and 1516.0 μg/g,respectively. This acidic method has advantages such as high extraction efficiency,low chemical toxicity and no special requirement of instruments. Therefore,it might be a more feasible and practical method for industrial practice.
【Fund】: Project supported by the National Natural Science Foundation of China (No. 20702019);; the Foundation for Young Professors of Jimei University China
【CateGory Index】: TQ920
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