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《浙江大学学报B(生物医学、生物工程版)(英文版)》 2008-01
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Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue (MIL-C) by Streptoverticillium rimofaciens ZJU 5119

Dan YE,Zhi-nan XU,Pei-lin CEN (Department of Chemical and Biochemical Engineering,Zhejiang University,Hangzhou 310027,China)  
Cytosine-substituted mildiomycin analogue (MIL-C) was produced effectively by supplementing cytosine into the culture of Streptoverticillium rimofaciens. In order to improve the yield of MIL-C,statistically-based experimental designs were applied to optimize the fermentation medium for S. rimofaciens ZJU 5119. Fifteen culture conditions were examined for their significances on MIL-C production using Plackett-Burman design. The Plackett-Burman design and one-variable-at-a-time design indicated that glucose and rice meal as the complex carbon sources,and peanut cake meal and NH4NO3 as the complex nitrogen sources were beneficial for MIL-C production in S. rimofaciens ZJU 5119. The results of further central composition design (CCD) showed that the optimal concentration of glucose,rice meal and peanut cake meal were 18.7 g/L,64.8 g/L and 65.1 g/L,respectively. By using this optimal fermentation medium,the MIL-C concentration was increased up to 1336.5 mg/L,an ap-proximate 3.8-fold improvement over the previous concentration (350.0 mg/L) with un-optimized medium. This work will be very helpful to the large-scale production of MIL-C in the future.
【Fund】: Project supported by the Ministry of Science and Technology China (No. 2004BA308A22-14);; the Department of Science and Tech- nology of Zhejiang Province China (No. 011102543)
【CateGory Index】: TQ920
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