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Developmental characteristics and response to iron toxicity of root border cells in rice seedlings

Cheng-hua XING1,Mei-hong ZHU2,Miao-zhen CAI2,Peng LIU2,Gen-di XU2,Shao-hui WU2(1Bioengineering Institute,Jinhua College of Profession and Technology,Jinhua 321007,China)(2College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua 321004,China)  
To investigate the Fe2+ effects on root tips in rice plant,experiments were carried out using border cells in vitro.The border cells were pre-planted in aeroponic culture and detached from root tips.Most border cells have a long elliptical shape.The number and the viability of border cells in situ reached the maxima of 1600 and 97.5%,respectively,at 20~25 mm root length.This mortality was more pronounced at the first 1~12 h exposure to 250 mg/L Fe2+ than at the last 12~36 h.After 36 h,the cell viability exposed to 250 mg/L Fe2+ decreased to nought,whereas it was 46.5% at 0 mg/L Fe2+.Increased Fe2+ dosage stimulated the death of detached border cells from rice cultivars.After 4 h Fe2+ treatment,the cell viabilities were ≥80% at 0 and 50 mg/L Fe2+ treatment and were 62% at 150,250 and 350 mg/L Fe2+ treatment;The viability of border cells decreased by 10% when the Fe2+ concentration increased by 100 mg/L.After 24 h Fe2+ treatment,the viabilities of border cells at all the Fe2+ levels were 65%;The viability of border cells decreased by 20% when the Fe2+ concentration increased by 100 mg/L.The decreased viabilities of border cells indicated that Fe2+ dosage and treatment time would cause deadly effect on the border cells.The increased cell death could protect the root tips from toxic harm.Therefore,it may protect root from the damage caused by harmful iron toxicity.
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