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《Journal of Zhejiang University(Engineering Science)》 2001-01
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Research on fermentation conditions for hEGF production in recombinant Escherichia coli

XU Zhi-nan, LIU Gang, CEN Pei-lin\;(Institute of Bioengineering, Zhejiang Uni., Hangzhou 310027, China)  
The effects of fermentation conditions on hEGF expression in recombinant \%E.coli\% strain JLU were examined in shake flask scale. It was found that initial glucose concentration and temperature were two key factors which affected hEGF expression. Maximum hEGF expression was observed when initial glucose was 5.0 g/L and the temperature was 32℃. Besides, high hEGF productivity was obtained when initial pH was adjusted between 6.6 and 7.3, and medium volume was 30 mL in 250 mL flask. The hEGF productivity could be further raised by 15.5% when 5 g/L glucose was replaced by 0.5 g/L glycerol as the carbon source in the fermentation medium.
【Fund】: 浙江省科学技术委员会国际合作基金资助项目 ;; 浙江大学曹光彪高科技发展基金资助项目
【CateGory Index】: TQ92
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