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《Journal of Zhejiang University(Natural Science)》 1999-01
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Researching the method of HPLC for analysing cis trans isomers of β carotene in Dunaliella saline

WANG Yong(Dept.of Biological Sciences and Technology,Zhejiang Univ.Hangzhou 310027,China)  
Carotenoids have big family in which β carotene have mang different stereoisomers.Our experiment using high performance liquid chromatography to analyse the cis trans isomers of Dunaliella salina β carotene,in order to get the optimun analylical conditions.We use WATERS HPLC system to get suitable conditions:1.Chromatographic columns:C 18 (250×4.6,Nucleosi 7μm);Mobile phase:Methanol∶Trichloromethane=60∶40( V/V );Flow rate:1 mL/min;detection,λ=436nm;temperature at 0~10℃.2.Two kinds of cis isomers and trans isomers are found.3.All of the experiments are in the conditions avoiding high temperature,stong,light,low pH.
【Fund】: 国家自然科学基金 浙江省自然科学基金
【CateGory Index】: Q562
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【References】
Chinese Journal Full-text Database 2 Hits
1 JIANG Jian-guo,ZHU Yue-hui,FANG Ke-teng(College of Food and Biology Engineering, South China University of Technology, Guangzhou 510640,China);HPLC Analysis of Carotenoids in Dunaliella bardawil and Its Comparison with Dunaliella Salina[J];食品科学;2004-05
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【Co-references】
Chinese Journal Full-text Database 7 Hits
1 ZHANG Hui-yong, LIU Guang-fa, LIN Hui-xin, TAN Jing(The Key Lab. of Ministry of Edu. for Cell Bio. and Tumour Cell engineering, School of Life Sci., Xiamen Univ., Xiamen 361005, China);RAPD Analysis in the Genus Dunaliella (Chlorophyte)[J];厦门大学学报(自然科学版);2001-06
2 JI Hong wu 1, SHI Zhao peng 2 (1.School of Food Science and Technology, Wuxi University of Light Industry, Wuxi 214036, China; 2.Hunan Agricultural University , Changsha 410128, China);HPLC Separation and Determination of Carotenoids in Ipomoea batatas[J];无锡轻工大学学报;2001-03
3 Li Shuqing,Suo Quanling,Yang Wei ( Department of Chemical Engineering,Inner Mongolia Polytechnic University ,Hohhot 010062) [HJ3/8];ANALYSIS AND DETERMINATION OF THE CAROTENOIDS IN JILANTAI DUNALIELLA SALINA BY HPLC[J];天然产物研究与开发;2000-06
4 WANG Yong(Dept.of Biological Sciences and Technology,Zhejiang Univ.Hangzhou 310027,China);Researching the method of HPLC for analysing cis trans isomers of β carotene in Dunaliella saline[J];浙江大学学报(自然科学版);1999-01
5 Wang Qiang, Han Yashan and Dai Yunqing (Dept. of Food Sciences, China Agricultural University, Beijing, 100094) Saishi Hirota (Dept. of Veterinary Medicine, Japan University, Japan);Simultaneous Determination of Five Carotenoids in Tomato by Reversed Phase High Performance Liquid Chromatography[J];色谱;1997-06
6 Ji Hongwu 1, Wang Zengsheng 2, Shi Zhaopeng 2 (1 Xianning Education College of Hubei, Wenquan 437100; 2 Hunan Agricultural University, Changsha 410128);Quantitative Analysis of Tea Carotenoids by HPLC[J];茶叶科学;1997-02
7 Jiang Jianguo Yao Ruhua (Dept. of Bioengineering,South China Univ.of Tech. Guangzhou 510641);ANALYSIS OF THE BIOCHEMICAL COMPOSITIONS AND THE β-CAROTENE ISOMERS IN FIVE SPECIES OF DUNALIELLA[J];华南理工大学学报(自然科学版);1997-10
【Secondary References】
Chinese Journal Full-text Database 5 Hits
1 Sun Xiejun;Li Xiuxia;Li Jianrong;Li Jiao;Tian Xin;Tao Xiuchun;College of Chemistry, Chemical Engineering and Food Safety, Bohai University; Food Safety Key Lab of Liaoning Province; Engineering and Technology Research Center of Food Preservation, Processing and Safety Control of Liaoning Province;;Optimization of Extraction and Saponification Technique of β-Carotene from Dunaliella salina[J];中国食品学报;2014-11
2 LUO Guang-hong;WANG Qin;YUAN Xiao-long;ZU Ting-xun;Kaiyuan Bio-tech Development Center,Microalgae Engineering Research Center of Gansu Province,Hexi University;Zhangye Institute for Food and Drug Control;Zhangye Medical College;;Determination of β-Carotene in Spirulina by RP-HPLC[J];食品科学;2013-22
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