Experimental study of UAG、bFGF and G-CSF in Treatment of Ischemia Hindlimb in Diabetic Rats
Zhang Lihai,Wang Jiao,Zhang Yajun,Jin Song,Chen Fujun*,Zhong Min,Wang Guimin,the First Hospital Affiliated to Jiamusi University,Jiamusi 154003,Heilongjiang Province,China
Objective This article aims at studying the effects of Unacylated Ghrelin(UAG) granulocyte colony-stimulating factor(G-CSF)and basic fibroblast growth factor(rh-bFGF) to induce vasculogenesis in ischemic hindlimb of the Diabetic Rat,probing its functional mechanism.Methods An ischemia hindlimb model was established both in health rats(n=60) and diabetic rats(n=60),which were divided into normal control group(n=20),bFGF + G-CSF group,and UAG + bFGF + G-CSF group respectively.The dose of UAG: 30umol/kg was injected into abdomen every other day.G-CSF(10ug/kg) was hypodermic injected for seven consecutive days.bFGF(5ug/kg) was intramuscular injected at days 1,3,5,and 7. MMP-9 in ischemia hindlimb muscle was examined by ELISA at a week after operation and the expression of CD34 in ischemia hindlimb muscle was examined by Immunohistochemistry at four weeks after operation.Results(1)Compared with N1,the expression of MMP-9 and quantity of Capillary in N2、N3 rats highly displayed(P0.01),but N2、N3 groups have no significant difference(P0.05);(2)In diabetic rats,each two groups have obvious difference(P0.01);(3)Compared health rats with diabetic rats,N1 vs D1 and N2 vs D2,have significant difference(P0.05).UAG group have no statistical significance.Conclusions bFGF combined with G-CSF can promote vasculogenesis in ischemic hindlimb rats.The ability of vasculogenesis in ischemic hindlimb of diabetic rats was significantly worse than normal rats.UAG combined with FGF,G-CSF can obviously promote vasculogenesis in ischemic hindlimb of diabetic rats.
【CateGory Index】： R587.1