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《Mdecular Cardiology of China》 2001-01
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Impact of Ryanodine Receptor Activation on Extracellularly Responsive Kinase / MitogenActivated Protein Kinase / Protein Kinase C Pathway in Cardiomyocytes

ZHU Zhiming, ZHU Shanjun, YANG Yongjian, et al. Department of Hypertension and Endocrinology, DapingHospital; Chongqing Institute of Hypertension, Third MilitaryMedical University, Chongqing 400042, China  
Objective Cardiac hypertrophy is regulated primarily by many hypertrophy-stimulating factors. However, little is known whether activation of ryanodine receptor (RyR) will effect on extracellularly responsive kinasc (ERK) / mitogen-activated protein kinase (MAPK) / protein kinase C (PKC) and cause cardiac hypertrophy. Methods primary neonatal rat cardiomyocytes were cultured in fetal calf serum (2%). Cytostolic free calcium concentration of cardiomyocyte was measured using ratiometric fura-2 fluorescence. DNA and protein synthesis of cardiomyocytes were performed by the 3H-thymidine and 3H-leucine incorporation. Western blotting evaluated ERK 1/2 protein expression. In addition, PKC and MAPK were immunoprecipitated, incubated with each specific substrates and [32P]-ATP, and their activities were measured by counting 32P-phosphorylated substrates. Ryanodine ( Ry) was used to stimulate RyR. Results Ry ( 1 nmol/L -1 μmol/L) cause a dose-dependent increase in cytostolic free calcium concentration of cadriomyocyte. RyR also promoted the DNA and protein synthesis of cardiomyocytes. ERK 1/2 expression of cardiomyocytes were significantly enhanced by administration of Ry. Stimulation of cardiomyocyt.es with Ry revealed an increase in activities of MAPK and PKC of cardiomyocytes, however, ruthenium red (RU), a specific inhibitor of RyR, suppressed Ry mediated the activation of PKC / MAPK Conclusions RyR also participated in modulation of cardiac hypertrophy though activation of ERK/MAPK/PKC signaling pathway besides its well known role in regulation of cardiac function.
【CateGory Index】: R541
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 ZHU Zhiming , ZHU Shanjun, HU Houxiang Hypertension Center, Daping Hospital, The Third Military Medical University, Chongqing 400042;Effect of knockout AT_(1a) receptor gene on transplasmamembrane calcium influx in aortic smooth muscle cells[J];NATIONAL MEDICAL JOURNAL OF CHINA;1999-09
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 ZHOU Yi-zhong,ZHU Shan-jun,YU Lin-jun,et al(Department of Cardiology,Xinqiao Hospital,The Third Military Medical University,Chongqing 400037,China);The effects of calcium influx induced by AngⅡ on PTEN expression[J];Editorial Department of Chinese Journal of Geriatric Cardiovascular and Cerebrovascular Disease;2005-04
2 ZHONG Jian, ZHU Zhi Ming , YANG Yong Jian (Department of Hypertension and Endocrinology and Chongqing Institute of Hypertension, Daping Hospital, The Third Military Medical University, Chongqing 400042);Inhibition of PKC and ERK1/2 in cultured rat vascular smooth muscle cells by angiotensin-(1-7)[J];Acta Physiological Sinica;2001-05
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