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《Molecular Cardiology of China》 2011-01
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Expression of osteopontin and cellular phenotype transformation in degenerative ascending thoracic aortic aneurysm

MENG Yan-hai1,TIAN Chuan1,LIU Lei1 WANG Liang1,LIU Wen-zhi1,CHANG Qian1.1Department of Cardiovascular Surgery, Cardiovascular Institute and FuWai Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing100037, China.  
Objective To study the expression of osteopontin(OPN) and investigate the role of OPN as well as phenotype transformation of vascular smooth muscle cells(vSMCs) in DATAA. Methods DATAA specimens were taken from 18 patients during elective surgical intervention, and 18 control specimens were obtained from patients who were undergoing coronary artery bypass surgery. Real-time PCR analysis was performed to quantify the mRNA expression level of OPN. Immunohistochemistry staining was performed to examine the expression and distribution of OPN protein. HE and Masson trichrome staining were performed to evaluate vSMCs arrangement and measure collagen content. Results Compared with controls, the protein expression levels of OPN in DATAA were significantly increased (P0.01). Similar result was observed in mRNA level of OPN (P0.01). The protein expression level of OPN was positively correlated with aortic diameter(r=0.737, P0.01). Using Masson staining method, we also observed elevated collagen area percentage[(33.11±4.10)% vs (17.31±3.94)%, P0.01] in the aortic media and adventitia of DATAAs, which was significantly correlated with the protein expression level of OPN (r=0.694, P0.01). Pathology results showed degradation of elastin and disordered arrangement of vSMCs in DATAA. Conclusions These results indicate that the expression levels of aortic collagen as well as OPN in DATAA specimens are increased. Synthetic-mode vSMCs may lead to increased secretion of collagen, which is likely to be responsible for the aortic wall pathological remodeling. VSMCs phenotypic transformation may play an important role in the pathogenesis and progression of DATAA.
【Fund】: 国家自然科学基金资助项目(81070252)~~
【CateGory Index】: R543.1
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