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《Molecular Cardiology of China》 2011-01
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Key Laboratory of Human Disease Comparative Medicine,Ministry of Heath

SHAO Hai-Tao ZHANG Li XIANG Zhi-Guang CHEN Wei ZHANG Xiao-Juan CAO Xing-Shui LV Dan, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences & Comparative Medical Center, Peking Union Medical College, Beijing 100021, China.  
Objective To generate the heart-specific cTnIR145G expression transgenic mice and to determine the function and morphology of the mice. Methods The transgenic vector was constructed by inserting the human cTnIR145G gene into the down stream of α-MHC promoter. The transgenic mice were created by the method of microinjection. The genotype of the transgenic line was identified by PCR and the expression level of the gene was determined by Western blot. The death was recorded and the pathologic changes were analyzed with echocardiography, electrocardiography and light microscopy observation. Reasults Four lines of C57BL/6J transgenic mice with high levels of cTnIR145G expression were established. The mice of high expressed line mostly dead before mature. The heart of cTnIR145G transgenic mice showed decreased ventricular chamber, increased ventricular wall and percent fractional shortening (FS%) compared with the non-transgenic mice by echocardiography analysis. Ventricular repolarization dysfunction was determined by electrocardiography. Myocardial hypertrophy and myocyte disarray were observed by histological analysis. Conclusions The cTnIR145G transgenic mice give a similar pathological phenotype with the human familial hypertrophic cardiomyopathy (FHC). The transgenic mice could be an useful animal model for the research of the mechanisms of mutation in cTnI caused FHC.
【Fund】: National Science and Technology Major Projects(2009ZX09501-026)
【CateGory Index】: R542.2
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【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 (Zhang Lei1,2,Sun Junhong2,Li Yongqiang3,Yu Yongmin1,Lu Jian2,Wang Yingyuan2/1.General Department of Criminal Police,Chongqing Public Security Bureau,Chongqing 400021;2.Shanxi Medical University School of Forensic Medicine,Taiyuan 030001,China;3.Department of Criminal Police,Changdu Public Security Bureau,Changdu,Tibet 854000);Advances in studies on skeletal troponin I and its prospective application in forensic medicine[J];Chinese Journal of Forensic Medicine;2013-04
2 SHI Wei-Li1,CHEN Yao2(1.Department of Cardiology,The Hospital of Chifeng City,Chifeng 024000 China;2.Pathological Physiology Teaching of Basic Medicine of Inner Mongolia Medical College,Huhhot 010050 China);Clinical Significance of Plasma Homocysteine (HCY) C-reaction Protein (CRP) Cardiac Troponin Ⅰ (cTnI) Level in Patients with Chronic Heart Failure[J];Inner Mongolia Medical Journal;2010-12
3 Yan Chen;Shiwei Yang;Jun Li;Gannan Wang;Yuming Qin;Daowu Wang;Kejiang Cao;Department of Emergency,the First Affiliated Hospital,Nanjing Medical University;Department of Cardiology,the First Affiliated Hospital,Nanjing Medical University;Department of Cardiology,Nanjing Children's Hospital,Nanjing Medical University;;Pediatric restrictive cardiomyopathy due to a heterozygous mutation of the TNNI3 gene[J];生物医学研究杂志(英文版);2014-01
4 Tao Qin1,Yang Junhua2 (1.Department of Cardiology,Jiangning Hospital of NanJing,NanJing 211100,JiangSu Province, China; 2.Department of Cardiology, First Hospital of SooChow University, Suzhou 215006 , JiangSu Province, China);Cardiac troponin I mutation in 14 Han nationality populations with familial hypertrophic cardiomyopath[J];Capital Medicine;2011-08
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