3-monochloropropane-1,2-diol induces oxidative DNA damage in HEK 293 cells

QU Hua-ling,JIANG Li-ping,YU Chang,et al.Department of Food Nutrition and Safty,Dalian Medical University(Dalian 116044,China)  

Objective The DNA damage induced by 3-monochloropropane-1,2-diol(3-MCPD) and the underlying mechanisms were studied in HEK293 cells.Methods As target cells,HEK293 cells were treated at the concentrations of 0,1.25,2.5,5,and 10 mM with 3-MCPD at 37 ℃ for 1 hr.The single cell gel electrophoresis assay(SCGE) was applied for quantitative analysis of DNA damage which was caused by 3-MCPD.We also measured the level of intracellular ROS by use of the 2,7-dichlorofluorescein diacetate(DCFH-DA) assay when HEK293 cells were treated with different concentrations of 3-MCPD(0,2.5,5,and 10 mM) at 37 ℃ for 1 hr.The level of oxidative DNA damage was evaluated using immunoperoxidase staining for 8-hydroxydeoxyguanosine(8-OHdG) when HEK293 cells were treated with different concentrations of 3-MCPD(0,1.25,2.5,5 mM) at 37 ℃ for 3 hr.Results The results showed that 3-MCPD at all tested concentrations(1.25-10 mM) caused a significant increase of DNA strand breaks in HEK293 cells.The numerical values for tail DNA% significantly increased compared to the control(P0.05).The level of intracelluar ROS was significantly increased when exposed to 10 mM of 3-MCPD(P0.05).The formation of 8-OHdG was significantly increased at the concentration of 2.5-5 mM of 3-MCPD(P0.05).Conclusion 3-MCPD can induce DNA damage in HEK293 cells.The DNA damage is probably induced by the increased level of intracellular ROS and formation of 8-OHdG,which cause oxidatively generated DNA damage and formation of DNA strand breaks.

【Key Words】： 3-monochloropropane-1 2-diol HEK293 cells DNA damage oxidative stress
【CateGory Index】： R114

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