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《Chinese Journal of Public Health》 2009-11
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3-monochloropropane-1,2-diol induces oxidative DNA damage in HEK 293 cells

QU Hua-ling,JIANG Li-ping,YU Chang,et al.Department of Food Nutrition and Safty,Dalian Medical University(Dalian 116044,China)  
Objective The DNA damage induced by 3-monochloropropane-1,2-diol(3-MCPD) and the underlying mechanisms were studied in HEK293 cells.Methods As target cells,HEK293 cells were treated at the concentrations of 0,1.25,2.5,5,and 10 mM with 3-MCPD at 37 ℃ for 1 hr.The single cell gel electrophoresis assay(SCGE) was applied for quantitative analysis of DNA damage which was caused by 3-MCPD.We also measured the level of intracellular ROS by use of the 2,7-dichlorofluorescein diacetate(DCFH-DA) assay when HEK293 cells were treated with different concentrations of 3-MCPD(0,2.5,5,and 10 mM) at 37 ℃ for 1 hr.The level of oxidative DNA damage was evaluated using immunoperoxidase staining for 8-hydroxydeoxyguanosine(8-OHdG) when HEK293 cells were treated with different concentrations of 3-MCPD(0,1.25,2.5,5 mM) at 37 ℃ for 3 hr.Results The results showed that 3-MCPD at all tested concentrations(1.25-10 mM) caused a significant increase of DNA strand breaks in HEK293 cells.The numerical values for tail DNA% significantly increased compared to the control(P0.05).The level of intracelluar ROS was significantly increased when exposed to 10 mM of 3-MCPD(P0.05).The formation of 8-OHdG was significantly increased at the concentration of 2.5-5 mM of 3-MCPD(P0.05).Conclusion 3-MCPD can induce DNA damage in HEK293 cells.The DNA damage is probably induced by the increased level of intracellular ROS and formation of 8-OHdG,which cause oxidatively generated DNA damage and formation of DNA strand breaks.
【Key Words】: 3-monochloropropane-1 2-diol HEK293 cells DNA damage oxidative stress
【CateGory Index】: R114
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【References】
Chinese Journal Full-text Database 1 Hits
1 ZHU Guo-ying1,XU Xiao-min2,GUAN Jian1(1.Jiaxing Center for Disease Control and Prevention,Jiaxing 314050,China;2.Zhejiang Center for Disease Control and Prevention,Hangzhou 310009,China);Rapid determination of 3-monochloropropane-1,2-diol in soy sauce by using gas chromatography-mass spectrometry[J];Chinese Journal of Health Laboratory Technology;2010-05
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 FU Wu-sheng1,WU Yong-ning2 (1.Fujian Center for Disease Control and Prevention, Fuzhou 350001, China;2.National Institute of Nutrition and Food Safety, Chinese Center for Disease Prevention and Control, Beijing 100050, China);Review on Analysis of Chloropropanols in Foodstuffs[J];Food Science;2007-03
2 Fu Wusheng Wu Yongning Zhao Yunfeng Ma Jinbo Zhang Qi (National Institute for Nutrition and Food Safety, Chinese CDC, Beijing 100050);Determination of dichloropropanols and monochloropropanediols in soy sauce by capillary gas chromatography/ion trap mass spectrometry using stable isotope dilution technique[J];Chinese Journal of Food Hygiene;2004-04
3 Ma Jin-bo1,Zhang Qi1,Luan Yan1,Yu Jun1,Yang Li-li2(1.Shenyang Center for Disease Control and Prevention,Shenyang 110031,China;2.Dongling Center for Disease Control and Prevention,Shenyang 110015,China);Determination of dichloropanols and monochloropanediols in soy sauce by MSPD-GC-MS using stable isotope dilution technique[J];Chinese Journal of Health Laboratory Technology;2007-04
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