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《China Poultry》 2018-07
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Comparative Analysis on HA Protein of H7N9 Subtype Influenza Virus with Different Pathogenicity

ZHANG Ying;LIU Lin;XU Haifeng;ZENG Xianying;SHI Jianzhong;WANG Xiurong;CHEN Hualan;Animal Influenza Laboratory of Ministry of Agriculture,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences;  
In order to clear the difference of serological characteristics of hemagglutinin(HA) between low-pathogenicity and high-pathogenicity H7N9 subtype influenza virus, HA proteins of two influenza viruses were expressed in vitro, and analyzed. HA gene was amplified by RT-PCR, sequenced, and cloned into the eukaryotic expressive vector pCAGGS-Flag to construct two recombinant expression plasmids, pCAF-H7HPHA and pCAF-H7LPHA. Recombinant expression plasmids were transfected into 293T cell. The expression of HA proteins were measured by indirect immunofluorescence and western blot. The results showed that continuous amino acid insertion was only found at the cleavage site of HA gene of high pathogenicity H7N9 subtype influenza virus, but not in the low-pathogenicity H7N9 subtype influenza virus. There were no differences in glycosylation sites and receptor-binding sites between the two H7N9 by influenza viruses. HA proteins were stably expressed, and the molecular weight was 70 ku. The HA proteins(or serum) of high-pathogenicity H7N9 subtype influenza virus could react with low-pathogenicity H7N9 subtype influenza virus serum(proteins). These results suggested that serological characteristics of HA protein from different pathogenic H7N9 subtype influenza virus were not different, which provided a basis for analysis on HA protein of H7N9 subtype influenza virus.
【Fund】: 国家重点研发计划项目(2016YFD0500800)
【CateGory Index】: S852.65
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