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《Chinese Journal of Rehabilitation Medicine》 2006-04
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Long-term adhere-wall culture and identification of human embryonic neural stem cell

ZHU Chang,ZHANG Chuanhan,LIU Zhiheng,et al. Dept. of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030  
Objective:To establish the method of long-term adhere-wall culture and passage of human embryonic neural stem cell.Method:By means of different culture methods, neural stem cells were isolated from human miscarriaged embryonic brain (embryonic age 9 weeks and 16 weeks). The capabilities of self-renew?nestin expression and multipotentiality of the neural stem cells were isolated and identified with immunocytochemical staining.Result:Human embryonic neural stem cell with the capabilities of self-renewing and multipotentiality can be isolated from the brain or subependyma of human embryo at different embryonic age by high concentration suspension culture. Neural stem cells possessed their characters and proliferated for 4 months stably by adhere-wall culture.Conclusion:Human embryonic neural stem cell can be isolated from human embryo of different embryonic age successfully. Human embryonic neural stem cells cultured by adhere-wall method can proliferate stably for a long time, which serve as a good model for transgenic research of human embryonic neural stem cell.
【Fund】: 国家自然科学基金资助项目(30300328)
【CateGory Index】: R329
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