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《Chinese Journal of Rehabilitation Medicine》 2006-06
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Up-expression of dynorphin A aggravates inflammatory edema induced by formalin test in rats

QI Wenxiu,LU Chongrang School of TCM, Southern Medical University;Postdoctoral station,Guangzhou TCM University;Guangzhou, 510515  
Objective: We utilized intrathecal(i.t.) application antisense oligodeoxynucleotide (AS-ODN) to induce prodynorphin (Pro-Dyn) mRNA and observed the degree of inflammatory edema after the formalin injection in rats to prove whether the quantity of dynorphin A in nerve centre could effect the degree of periphery inflammation.Method: The rats were pretreated with i.t. application of AS-ODN, normal saline(NS) or the reverse ODN(RS-ODN) separately. The rat′s paw was injected with formalin and observed the incremental percentages of diameter difference(%) combined with the immunocytochemical evidences that intensified expression of dynorphin A(1-8)(Dyn A)(OD units) in spinal dorsal horn after the formalin injection in 1h,2h and 3h time slices.Result:①All the injected paws showed inflammatory edema with a progressive aggravation in three inspect times after the formalin injection, at the same time interval. The formalin induced incremental percentages of diameter differences of injected paws in i.t. AS-ODN group were smaller than that in i.t NS or i.t RS-ODN group but there were no differences in i.t NS group and in i.t RS-ODN group. ②The up-expression of Dyn A in ipsilateral dorsal horn to the formalin injection increased gradually follow the formalin injection in all groups of all inspect times, and there were markedly reducer in i.t. AS-ODN group than that in i.t NS or i.t RS-ODN group but there were no differences in i.t NS group and i.t RS-ODN group in all inspect times.Conclusion: Application of AS-ODN induced prodynorphin mRNA could suppress the increase expression of Dyn A in the spinal dorsal horn along with prevention on the local inflammatory edema.
【CateGory Index】: R363
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Chinese Journal Full-text Database 2 Hits
1 Zhang Ruixin; Chen Jinyan; Maryann A; Ruda; Qiao Jiantian(Department of Physiology, Shanxi Medical University, Taiyuan; Pain and NeurosensoryMechanisms Branch, National Institute of Dental Research, National Institutes ofHealth, 9000 Rockville Pike, Bethesda, MD 20892);FUNCTIONAL RELATIONSHIPS BETWEEN PERIPHERAL INFLAMMATION,THERMAL HYPERALGESIA, AND ENHANCED DYNORPHIN GENE EXPRESSION IN SPINAL CORD OF RAT: BEHAVIORAL AND mRNA STUDIES[J];Chinese Journal of Neuroanatomy;1999-01
2 Qi Wenxiu, Zhang Yu *, Qi Jinshun *, Qiao Jiantian * (Department of Physiology, Fenyang College, Shanxi Fenyang 032200; *Department of Neurobiology, Shanxi Medical University, Taiyuan 030001);INTRATHECAL ADMINISTRATION OF PRODYNORPHIN ANTISENSE OLIGODEOXYNUCLEOTIDE SUPPRESSES BOTH PRODUCTION OF DYNORPHIN A IN DORSAL HORN AND BEHAVIORAL NOCICEPTIVE RESPONSES INDUCED BY FORMALIN TEST[J];Chinese Journal of Neuroanatomy;2003-04
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