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Effects of Three Elicitors on PPO and POD Activities in Ramie Leaves

AN Xiao-xia;ZENG Liang-bin;YU Yong-ting;YANG Rui-lin;Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences;  
Polyphenoloxidase( PPO) and peroxidase( POD) are important for plant resistance and can be induced by elicitors.In this paper,three elicitors( Salicylic Acid,Chitosan oligosaccharide and Methy jasmonate) with different concentrations were sprayed on the seedlings of ramie ″Chuanzhu No.8″,and then PPO and POD activities at different time were monitored.The elicitor with a certain concentration which could stimulate the highest increase of enzyme activities was regarded as the suitable one.The results showed that these three elicitors could all raise enzyme activities of PPO and POD.Especially 0.8mM Salicylic Acid,3g / L Chitosan oligosaccharide and 1mM Methy jasmonate could induce higher enzyme activities than other concentrations of the same elicitors.Additionally,the peak of both the enzyme activities appeared around 7-8d after spraying 0.8mM Salicylic Acid,and the effects were more stable than the other two elicitors.Therefore,Salicylic Acid was selected as a better elicitor for inducing ramie resistance,0.8 mM of concentration and spraying 7d early could get a good result.
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