Optimization of Extraction Technology of Hemp Protein by Response Surface Method
LIU Shuxia;GUO Yongxia;WEI Guojiang;HAN Chengwei;DU Chunyu;PAN Dongmei;XU Haijun;GUAN Xiangjun;WANG Xiaofei;GUO Mengqiao;Daqing Branch of Heilongjiang Academy of Sciences;Heilongjiang Bayi Agricultural University;Daqing Green Agricultural Products Monitoring Center;
The traditional method of alkali extraction and acid precipitation was used to gain the protein of "Hemp seed No. 1 "in this paper. Four factors were taken into account,including extracting time,emperature,ratio of liquid to solid and pH value. Meanwhile the single factor tests and response surface experiment were designed to get the optimal combination for the max extraction ratio of protein.The result showed that under the optimized condition of extracting time 1. 58 h,extracting temperature at49. 54℃,pH 10. 99,ratio of liquid to solid 20. 17: 1,the protein yield could reach 25. 01%. It also indicated that "Hemp seed No. 1"had high content of protein,which would be of great value for futher development and utilization.