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《China Leather》 2017-01
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Determination methods of protease activity of fur bating wastewater

WANG Yaping;CHENG Fengxia;XUE Xuntian;XU Gede;LU Jian;HUANG Yunxia;College of Material and Textile Engineering,Jiaxing University;Tongxiang Xinnuo Fur Co.,Ltd.;  
Determination of enzyme activity is the basis of realizing the fur bating wastewater recycling. The effect of sodium chloride,amino acid on protease activity was studied based on the components and characteristics of fur bating wastewater. The method of "dialysis pretreatment- forint phenol testing the enzyme activity of bating wastewater"was put forward by optimizing the testing conditions of dialysis pretreatment and enzyme activity based on the testing theory and characteristics of protease activity of current forint phenol method. Results show that the sodium chloride and tyrosine in bating wastewater can severely impact the testing results,needing to be excluded. Recoveries of the dialysis pretreatment- forint phenol method range from 100. 0% to 110. 0%,which can basically meet the requirements of industrial determination. The research achievements have practical guiding significance in enzyme activity ration of recycling fur bating wastewater.
【Fund】: 浙江省教育厅一般科研项目(项目编号Y201534280)
【CateGory Index】: TS522
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