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《Acta Laboratorium Animalis Scientia Sinica》 2001-04
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Analysing DNA Fingerprints of Inbred Rat Strains

LI Ruisheng, CHEN Zhenwen, WANG Chengli, LUAN Ronghui (Laboratory Animal Center, Academy of Military Medical Sciences, Beijing 100071,China)  
Objective In order to set up the method of DNA fingerprints in the heredity monitoring of inbred rats. Methods\ National nine groups of seven strains were analysed using DNA fingerprints, and the same DNA were repeatedly tested by DNA fingerprints. Results\ (1)There were great diversity of DNA fingerprints in various strains, whose average bands were 16 360±2.178,band sharing level was 0.061±0.008,and the similarity coefficient was 0.062±0.008,the probability of an identical DNA fingerprints in them was 3.691×10 -23 . (2) Except that the similarity coefficient and band sharing level of SHR(Haerbin)and WKY(Haerbin)were less than 0.7 in various individuals of the same group, the remainder were more than 0.9. (3) It was diverse between the same inbred SHR strains or WKY strains in different areas, the similarity coefficient and band sharing level being less than 0.6. (4) DNA fingerprints from the same DNA were almost consistent in repeated experiments ( P 0 05). Conclusions\ Feasibility of DNA fingerprints in the heredity monitoring of inbred strain rat was proved.
【Fund】: 总后卫生部基金课题资助 ( 98M116 )
【CateGory Index】: Q95-331
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