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《Acta Laboratorium Animalis Scientia Sinica》 2001-04
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Effect of Recombinant Human Luteinizing Hormone on the Development of Murine Oocytes Fertilized in vitro

GUO Yong, XIA Guoliang, LEI Lei, BU Shumin, XIE Huirong (College of Biology, China Agricultural University, Beijing 100094,china)  
Objective To investigate the effect of r hLH on the development of murine oocytes fertilized in vitro. Methods The prepubertal immature Kunming White mice were superovulated by r hFSH alone(20 IU), r hFSH+r hLH(20 IU each) or r hLH alone(20 IU). Their absorbed cumulus enclosed oocytes (CEO) were fertilized in vitro. These IVF murine preembryos were also in vitro cultured and observed.Results The IVF rate of the group with both recombinant gonadotropins(20 IU r hFSH+ 20 IU r hLH), was significantly higher than that of the group with one of them alone(either 20IU r hFSH or 20 IU r hLH, 72 2%∶37.2% or 39.9%), but the 16~32 cell rate with r hFSH(20 IU) alone was significantly higher than that of the other two groups (33.01%∶1.75% or 0).Conclusion r hLH could improve the fertility of murine oocytes, but affect their further development in vitro cultured (IVC preembryos) negatively.
【Fund】: 国家杰出青年基金 ( 30 0 2 5 0 32 )
【CateGory Index】: Q954.4
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