Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Laboratorium Animalis Scientia Sinica》 2001-04
Add to Favorite Get Latest Update

The Study of an Animal Fever Model Using Endotoxin and Its Standardization in Rabbits

YANG Guojie, CHEN Xiulian, SHAO ping, TIAN Feng, KANG Aijum, YU Fang (Department of Laboratory Animal Science, Health Science Center, Peking University, Beijing\ 100083, China)  
Objective To determine the effect from different doses of LPS,the temperature and humidity of the ambient, rabbits restraining conditions and physical functions on rabbits febrile response.Method Intravenous application of endotoxin (ET) is a traditional method to construct an animal fever model.The key factor is to find the optimum dosage.The fever was studied after different doses of ET were intravenously injected (Ⅳ). Results and conclusion the results of the study showed:1)The increase of LPS dosage had a promotive effect on the febrile response of the rabbits with shorter latency to onset,higher peak fever change, longer duration and greater temperature response index (TRI).2) The rabbits developed a positive dose\|dependent fever in response to ET over the range of 0.5?ng/kg\|2000?ng/kg.Small doses (0.5?ng/kg,2?ng/kg)of ET induced a monophasic fever and large doses (20?ng/kg,100?ng/kg,200?ng/kg,2000?ng/kg)of ET induced a biphasic fever.In response to the doses of 100?ng/kg and 200?ng/kg the rabbits developed a homogeneously typical biphasic fever.Therefore,the recommended range of ET dose is from 100?ng/kg to 200?ng/kg in making a rabbit fever model.The results shows that (25±1)℃ ambient temperature is better than (18±1)℃ and the neck stock is better than the prone stock.
【Fund】: 北京市科学技术委员会专项资金资助项目( 95 49815 0 0 )
【CateGory Index】: R-332
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 ZHOU Qin1,FU Ju-fang2,SUN Jing2,ZHANG Ming2,SHI Xue-Qing2,ZOU Xiao-mei1,GUAN Hao1(1.Department of Burns & Skin Surgery,First Affliated Hospital,the Fourth Military Medical University,Xi'an 710032,China;2.Department of Nursing,First Affliated Hospital,the Fourth Military Medical University,Xi'an 710032,China);Study on Anti-pyretic Effects of Intravenous Dripping of Hypothermal Solution on Fever Rabbit with 30% TBSA Deep Partial Thickness Scald[J];Nursing Journal of Chinese People's Liberation Army;2008-22
China Proceedings of conference Full-text Database 1 Hits
1 Qiu Ze-wen1, Gao Wen-ting1, Wang Xi1, Li Hong-xia1, Han Xu1,Chen Zhi-min2, Wang Jing-yu1 (1.Dalian medicine university, Dalian 116027; 2.Liaoning tianyu medicine trade limited company);Research on increasing the utility rate of the rabbits in the heat source test[A];[C];2006
Chinese Journal Full-text Database 10 Hits
1 CHEN Geng-fu,GUO Xiao-ling,MENG Qing (Guangzhou College of Pharmacy,Guangzhou 510224);Study on Chemical Constituents of Dry Zingiber[J];Amino Acids & Biotic Resources;2002-02
2 LI Gao-kai,LIANG Xue-wu~*,LIU Qing-hua(Dairy Cattle Institute of Fujian Agricultural and Forestry University,Fuzhou,Fujian 350002,China);The Preliminary Study on Effect and Mechanism of Heat Stress Alleviators for Dairy Cows[J];China Cattle Science;2006-03
3 Ma Jian Meng Shujiang (Nanjing College of TCM, Jiangsu);An Experimental Research on Qi-ying Phase Transformation Blocked by Detoxifying, COoling Ying and protecting Yin[J];;1991-01
4 Edwin A Deitch Ma Li, et al (Burn Research Institute, Southwest Hospital, Third Military Medical College) Edwin A Deitch Edwin A.Deitch Edwin A Deitch;AN EXPERIMENTAL STUDY OF THE BACTERIAL TRANSLOCATION FROM GUT[J];Acta Academiae Medicinae Militaris Tertiae;1990-01
5 LI Zi-jiao,YANG Yi (School of Science, Beijing University of Chemical Technology,Beijing 100029), DING Ming-yu~* (Department of Chemistry, Tsinghua University, Beijing 100084), ZHAO Zhong-zhen (School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tang, Hong Kong) and LIU De-lin (Institute of Basic Theory, China Academy of Traditional Chinese Medicine, Beijing 100700), Fenxi Shiyanshi, 2005, 24(4): 67~69;Analysis of flavonoids, alkaloids and organic acids in the non-ephedrine alkaloids of the extract of Ma Huang[J];Analytical Laboratory;2005-04
6 ZHENG Jia-keng\+1,DAI Jin-cheng\+1,YANG Jun-lian\+2,et al\;(1.Dept.of Traditional Chinese Medicine,FCTCM,Fuzhou,Fujian 350003,China; 2.Singapore College of Traditional Chinese Medicine) \;;Experimental Study on Effects of modified RENSHENBAIHU Decoction to Blood Sugar and Immunological Function in Diabetes Rats[J];Journal of Fujian College of Traditional Chinese Medicine;2001-01
7 DAI Jin cheng,ZHEN Jia keng,HUANG Jing xin,et al\;(Dept.of Traditional Chinese Medicine,FCTCM,Fuzhou,Fujian 350003,China) \;;Experimental Study of RENSHEN BAIHU Decoction on Models of Diabetic Rats[J];Journal of Fujian College of Traditional Chinese Medicine;2001-03
8 WU Yu-ming1,HE Xuan-hui1,3,ZOU Yu-xiao1,ZHANG Rui-fen1,LIAO Sen-tai1, LIU Xue-ming1,YE Yun-shou2,HUANG Qing-hua2(1.Sericultual Farm Produce Processing Institute,Guangdong Academy of Agricultual Sciences/ Guangdong Open Laborartory of Farm Produce Processing,Guangzhou 510610,China;2.Animal Academy of Siences,South China Agricultural University,Guangzhou 510640,China3.Donguan Starway Biological Ltd.,Dongguan 523722,China);Study on the immunodulatory effect of Zhenjunshu[J];Guangdong Agricultural Sciences;2008-02
9 Lv Yi,Wang Haibin,Hu Sen,et al.Burn Institute,First Affiliated Hospital of General Hospital of PLA,Beijing,100037,China;Excitation of the cholinergic pathway protects liver from injury in endotoxemia and gut ischemia/reperfusion animals[J];Infection,Inflammation,Repair;2007-02
10 Zhang Jian yong;Animal Experimental Research of Wen Bing Xie Re Yong Fei Syndrme[J];Journal of Gansu College of Traditional Chinese Medicine;1990-01
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 XIANG Qing-jun,LI Ce-sheng,Hu Yong,et al.(Wuhan Institute of Biological Products,Wuhan 430060,China);Effect of the different basal body temperature of rabbits on pyrogen test results[J];Progress in Microbiology and Immunology;2009-04
2 TAN De-jiang DU Ying ZHANG Xiao LI Guan-min (National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050, China);Disscussion of the major differences of pyrogen test between USP、EP 、JP and ChP[J];Chinese Journal of Pharmaceutical Analysis;2008-12
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved