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《Acta Laboratorium Animalis Scientia Sinica》 2001-04
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The Study of an Animal Fever Model Using Endotoxin and Its Standardization in Rabbits

YANG Guojie, CHEN Xiulian, SHAO ping, TIAN Feng, KANG Aijum, YU Fang (Department of Laboratory Animal Science, Health Science Center, Peking University, Beijing\ 100083, China)  
Objective To determine the effect from different doses of LPS,the temperature and humidity of the ambient, rabbits restraining conditions and physical functions on rabbits febrile response.Method Intravenous application of endotoxin (ET) is a traditional method to construct an animal fever model.The key factor is to find the optimum dosage.The fever was studied after different doses of ET were intravenously injected (Ⅳ). Results and conclusion the results of the study showed:1)The increase of LPS dosage had a promotive effect on the febrile response of the rabbits with shorter latency to onset,higher peak fever change, longer duration and greater temperature response index (TRI).2) The rabbits developed a positive dose\|dependent fever in response to ET over the range of 0.5?ng/kg\|2000?ng/kg.Small doses (0.5?ng/kg,2?ng/kg)of ET induced a monophasic fever and large doses (20?ng/kg,100?ng/kg,200?ng/kg,2000?ng/kg)of ET induced a biphasic fever.In response to the doses of 100?ng/kg and 200?ng/kg the rabbits developed a homogeneously typical biphasic fever.Therefore,the recommended range of ET dose is from 100?ng/kg to 200?ng/kg in making a rabbit fever model.The results shows that (25±1)℃ ambient temperature is better than (18±1)℃ and the neck stock is better than the prone stock.
【Fund】: 北京市科学技术委员会专项资金资助项目( 95 49815 0 0 )
【CateGory Index】: R-332
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