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《Acta Laboratorium Animalis Scientia Sinica》 2018-05
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Efficacy of Du Zhong injection on lipopolysaccharide-induced mastitis in mice

SHAN Xufei;WANG Jianfa;LI Qitao;HE Xianjing;HAO DANDan;ZHANG Xu;WANG Hai;WU Rui;College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University;  
Objective In this study, we tested the therapeutic efficacy and mechanisms of action of Du Zhong injection in a mouse mastitis model. Methods The mastitis mouse model was produced by injection of lipopolysaccharide(LPS) into the breast duct. At 12 h post LPS injection, mice were split into six groups and administered different concentrations of Eucommia ulmoides(Du Zhong) into the abdominal cavity. After a further 12 h recovery, mice were killed and the mammary glands collected. Pathological changes in each group were observed using hematoxylin and eosin staining. Changes in tumour necrosis factor-α(TNF-α), interleukin-6(IL-6) and interleukin-1(IL-1) cytokines in breast tissue were detected by enzyme-linked immunosorbent assay. Results The mouse mastitis model was successfully constructed using 50 μL of 0.2 mg/ml LPS. Pathological examination revealed that, increasing doses of Du Zhong injection were associated with decreased mammary gland injury in the model mice, with the greatest effect observed at 20 mg/kg and 27 mg/kg. By enzyme-linked immunosorbent assay, Du Zhong injection also reduced pro-inflammatory cytokine expression in the breast tissue, with greatest effects observed at 27 mg/kg(P 0.01). Conclusions Du Zhong injection can significantly reduce pathological damages, pro-inflammatory cytokine release, and neutrophil invasion in mammary gland tissue in a mouse model of mastitis, with an optimal dosing of 27 mg/kg.
【Fund】: 黑龙江八一农垦大学研究生创新科研项目(YJSCX2017-Y35);; 国家自然基金(2041340021);; 大庆市科技局(zd-2016-094)~~
【CateGory Index】: S853.74
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