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《Acta Laboratorium Animalis Scientia Sinica》 2018-05
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Delivery of anti-miR34c by polyamidoamine dendrimer attenuates hepatic fibrosis in nude mice

ZHANG Huanhuan;FANG Jie;YU Wenying;YANG Yang;YU Chenhuan;YING Huazhong;Zhejiang Academy of Medical Sciences Center of Laboratory Animals,Key Laboratory of Experimental Animal and Safety Research of Zhejiang Province;  
Objective Upregulation of miR34 in the liver has been found in numerous liver diseases, from fatty liver disease to hepatocellular carcinoma. In the present study, we examined the protective actions of anti-miR34 c polyamidoamine(PAMAM) dendrimer nanoparticles on hepatic fibrosis in nude mice. Methods Fifteen clean nude mice were randomly divided into the normal control, model control, and anti-miR34 c PAMAM dendrimer nanoparticles groups(n=5 per group). Hepatic fibrosis was induced by intraperitoneal injection of 100 μL of 20% carbon tetrachloride twice weekly for 6 weeks. Hepatic pathology, serum biochemical indicators, and transforming growth factor-β(TGF-β)/α-smooth muscle cell actin(α-SMA) immunohistochemistry were examined. Results Anti-miR34 c delivered by the PAMAM dendrimer decreased inflammatory cell infiltration, collagen deposition, and TGF-β and α-SAM expression in the liver, and improved serum biochemical factors. Conclusions Anti-miR34 c PAMAM dendrimer nanoparticles can improve hepatic fibrosis by reducing activation of HSC and incidence of liver injury in nude mice.
【Fund】: 国家自然科学基金(81573591);; 浙江省医药卫生科研项目(2015KYB091)~~
【CateGory Index】: R575.2;TB383.1
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