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《Acta Laboratorium Animalis Scientia Sinica》 2018-05
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Isolation, characterization and differentiation of ferret adipose-derived stem cells

SHI Guiying;LI Keya;XU Yanfeng;HAN Yunling;GAO Shuping;Laboratory of Stem Cells and Translational Medicine, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences & Comparative medical Center, Peking Union Medical College;  
Objective The objective of this study was to isolate, characterize, and differentiate ferret adipose-derived stem cells(ADSCs). Methods Subcutaneous fat tissues from ferret abdomens were obtained via germ-free procedures, mechanically minced into 0.5 mm~3 pieces, and digested with 0.075% collagenase. Subsequently, ADSCs were cultured in high-glucose DMEM medium containing 10% FBS, antibiotics, and 4 ng/mL β-FGF. Then, analyses of cell morphology and cell surface antigen expression were made by flow cytometry. Finally, osteogenic, adipogenic, chondrogenic, and neural differentiation were examined using different method. Results We isolated and identified ADSCs from fresh fat tissues of ferrets, which demonstrated a more fibroblast-like morphology. FACS analysis show high expression of CD29(53.1%), CD90(99.6%) and CD105(93.7%), but low expression of CD11 b(36.3%) and CD44(28.3%). The ADSCs could be differentiated into adipocytes, osteoblasts, chondrocytes, and neurons by in vitro induction. Conclusions ADSCs can be isolated from the subcutaneous fat tissue of ferrets, and these cells have multi-directional differentiation ability.
【Fund】: 协和青年科研基金项目(2017310018);; 医科院科技支撑项目(DWS201516);; 北京市重大科技项目(17E10002);; 2017国家干细胞与转化医学重点研发项目(2017YFA0105202)~~
【CateGory Index】: Q813
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