Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Laboratorium Animalis Scientia Sinica》 2018-05
Add to Favorite Get Latest Update

Effect of NF-E_2 related factor 2 pathway activation on PM_(2.5) induced reproductive injury in female rats

ZHANG Fengquan;ZHAO Shan;DONG Enheng;CAO Jincheng;GUO Xudong;Key laboratory for Air Pollution Health Effects and Intervention of Xinxiang, School of Public Health, Xinxiang Medical University;Analytical and testing Laboratory, School of Basic Medicine;  
Objective To study the effect of NF-E2 related factor 2(Nrf-2) pathway activation on particulate matter 2.5(PM_(2.5))-induced reproduction injury in female rats. Methods Thirty Sprague Dawley rats were randomly divided into control(normal saline), low dose exposure(1.5 mg/kg PM_(2.5)), and high dose exposure(37.5 mg/kg PM_(2.5)) groups. The levels of tumor necrosis factor-α(TNF-α), interleukin 1-β(IL-1β), and interleukin-6(IL-6) were measured by enzyme-linked immunosorbent assay. The mRNA and protein expression of Nrf-2 and heme oxygenase-1(HO-1) were also examined by qPCR and western blot. Results There was a significant increase in TNF-α, IL-1β, and IL-6 expression in the low dose and high dose exposure groups compared with the control groups(P0.05). The expression levels were also increased with increasing dose of PM_(2.5)(r=0.870, 0.847, and 0.855, respectively). Both the mRNA and protein expressions of Nrf-2 and HO-1 were also significantly increased in the low dose and high dose exposure groups compared with controls(P0.05). Conclusions The Nrf-2 signaling pathway is activated following exposure of PM_(2.5) in female rats, although PM_(2.5) can still induce uterine inflammation and injury.
【Fund】: 河南省高校科技创新团队支持计划(14IRTSTHN017)~~
【CateGory Index】: R122.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved