Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Laboratorium Animalis Scientia Sinica》 2018-05
Add to Favorite Get Latest Update

Combined behavioral test to detect delirium induced by scopolamine in mice

REN Quan;CHEN Xuhui;CHEN Ming;YANG Jianjun;Department of Anesthesiology, Zhongda Hospital Affiliated to Southeast University;Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology;Department of Urology, Zhongda Hospital affiliated to Southeast University;  
Objective To develop a combined behavioral test for assessment of scopolamine-induced delirium in mice. Methods Two groups of mice were randomly assigned to receive 15 mg/kg scopolamine(delirium group; n=12) or isovolemic saline(control group; n=8) via intraperitoneal injection. We developed a novel behavioral experiment in which mice received the light and black box test for 10 min, the open-field test for 10 min, and then the non-selective non-sustained attention test for 10 min, from 30 to 60 min after intervention. Five behavioral indexes were compared between the groups to assess the delirium status. Results Compared with the control group, there were significant differences in all five behavioral indexes in mice in the delirium group mice at 30 min after intervention: time spent in the white box was significantly shorter(P 0.05), the movement speeds were significantly increased(P 0.05), time spent beside the wall area was increased significantly(P 0.05), the freezing time, and the attention level were significantly decreased(P 0.05). Conclusions This combined behavioral experiment can be used to assess the key elements for delirium diagnosis, and with a faster time than conventional tests.
【Fund】: 江苏省卫计委面上项目(H2017005);; 中央高校基本科研业务费专项资金资助(2242017K40254)~~
【CateGory Index】: R-332;R749
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved