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Preperation of Anti Ectromelia Monoclonal Antibody by Intrasplenic Direct Immunization

He Zhengming,et al (National Institute for the Control of Pharmaceutical and Biological Products)  
Five hybridoma cell strains which could stably secrete McAbs to Ectromelia Virus were obtained by intrasplenic direct immunization and hybridoma techniques as those described in the paper,The titer of McAbs in culture supernatant and AF are 1∶8~64 and 10~10 seperately by ELISA Nonspecific reaction between McAbs and 10 murine viruses and BHK21cells was not observed.This method is a very practical and valuable one because it is one not only minimize the dose of antigen and shorten the immunizing time compared with common immunization method,but also avoid the failure of immunization with virulent vital antigen.
【CateGory Index】: R392-33
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【Secondary References】
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1 WANG Jing1,WANG Xing-xing1,2,GAO Fei1,YUAN Si-chun1,WU Guo-juan1(1.Department of Animal Science and Technology,Beijing University of Agriculture,Beijing 102206,China; 2.Beijing Sanyuan Luhe Dairy Farming Center,Beijing 100076,China);Procaryotic Expression and Purification of the Recombinant Nucleocapsid Protein of PRRSV and Preparation of the Monoclonal Antibody[J];中国畜牧兽医;2012-04
2 HUANG Yuan-yuan et al(College of Animal Science and Technology,Southwest University,Chongqing 400715);Research Progress of Porcine Reproductive and Respiratory Syndrome Virus[J];安徽农业科学;2012-11
3 LAN Hai-nan1,KONG Jin-chao2,ZHAO Xiao-li1,YAN Feng1,LI Wei1,ZHAO Xue1,GUO Feng1,ZHENG Xin1(1.College of Ainimal Science and Technology,Jilin Agricultural University,Changchun 130118,China;2.College of Agriculture,Yanbian University,Longjing 133400,China);Preparation of Monoclonal Antibody of PRRSV(CH-IR Strain)[J];中国畜牧兽医;2011-04
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