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Study on Techniques of Making Transgenic Mice by Microinjection Method

Ju Limei, et al (Institute of Laboratory Animal Science Chinese Academy of Medical Sciences,Beijing)  
ransgenic technique is one part of the development engineering or embryonic manipulation. This techniques include collecting embryos, in vitro culture of embryos and transferring embryos as a basic technology. Production of transgenic mice is one kind of application technology. The foreign DNA ware microinjected into nucle of fertilized one cell eggs and integrated into apparently random sit in the host genome. We produce transgenic mice by microinjection with NchW (Human prion protein gene),pCSLN(Sialidase-L gene),prbo(Bovine prion protein gene),CXTGAP(Human GM 2 activation protein gene),Prsh (Sheep prion protein gene) DNA fragments into the pronucleus of fertilized eggs. The DNA integrates on chromosomes at random and we got the positive result from the pups by PCR-Southern method. The average positive rate of transgenic mice is 16.38%. The present work proved that the main requirement of succeed on production transgenic mice should include precise technical skill, animal chosen and husbandry,manipulation
【Fund】: 国家重点资助项目
【CateGory Index】: Q785
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