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《Chinese Journal of Rice Science》 1987-02
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Isolation and Purification of mRNA and in vitro Translation for Rice(O.sativa)

Huang Danian (China National Rice Research Institute)  
The mRNA is a tool transferred genetic information for DNA and can be copied by reverse transcriptase to form a cDNA which can be used as a probe for molecular hybridization to isolate specific genes.At present selected young seedling of rice at 10 days to extract total mRNA.From 52.6mg fresh tissues got6.5mg total RNA.According toA260/A280=1.69,it indicated that a little amount protein was contained in RNA extracted.Applied poly(U)-sepharose column,88μg total mRNA was got from total RNA.The in vitro products for rabbit reticulocyte lysate translation system showed that isolated mRNA possessed biological function.
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