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《Chinese Journal of Rice Science》 1999-01
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Homozygous Lines of Transgenic Rice (Oryza sativa L.) Obtained via Anther Culture

SI Huamin; FU Yaping; XIAO Han; HU Guocheng; CAO Junping; HUANG Danian; SUN Zongxiu(1 Key Laboratory for Rice Biology, Ministry of Agriculture, P. R. China, China National Rice Research Institute, Hangzhou 310006;2 Department of Biotechnology, China National Rice Research Institute, Hangzhou 310006)  
Hundreds of anther--derived lines from transgenic rice plants with insertion of bar gene were obtained by a PAA based one-step method of anther culture. Inheritance and expression of transgene bar in 6 and 25 anther-derived lines from twoprimary transformants Jingyin 119--B3 and Jingyin 119--B4 respectively which were obtained with Biolistics were analyzed. bargene expressed normally in haploid and diploid plants as in their parents. PCR and Southern blot analysis of genomic DNAfrom some anther--derived lines indicated that bar gene was intact after anther culture and there was no change of integrationpatterns in most lines. Our results demonstrated that anther culture could be used for rapid production of transgene-homozygous lines from transgenic rice plants, even from transgenic plants with multi-locus insertion. System of transformation--anther culture-breeding and/or genetic study was also discussed.
【Fund】: 浙江省自然科学基金;;美国洛克菲勒基金会的资助
【CateGory Index】: S511
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