Molecular and Submicrostructural Research of Rice Seed Soaked in DNA Solution
LIU Chunlin; RUAN Ying; DONG Yanyu(1)Crop Engineering Key Laboratory of Hunan Province, Hunan Agricultural University, Changsha 4101281 ;2)Biological Department, ScienceCollege, Hunan Agricultural University, Changsha 410128)
Rice uncoated seeds were soaked in pBI121 plasmid solution, and screened in G418 solution. The rice seedlings resistant to G418 were got. Southern blot analysis indicates that NPT Ⅱ gene in pBI121 integrated into the genome of rice acceptor. Bymeans of scanning electronic microscope, the surface of cell in apical point show that there were different microstructures betweenmaterials soaked in DNA solution and controls, which means that special gene expression must have been induced by DNAmolecules.
【CateGory Index】： S511