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《Chinese Journal of Rice Science》 2003-01
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SSR Markers Selection and Purity Detection of Major Hybrid Rice Combinations and Their Parents in China

PENG Suo tang 1, ZHUANG Jie yun 2, YAN Qi chuan 1, ZHENG Kang le 2( 1 College of Agricultural and Biotechnology, Zhejiang University, Hangzhou 310029, China; 2Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China; 3Zhejiang Senhe Seed Co., Ltd, Hangzhou 310021, China)  
Twenty six SSR primers distributing in 12 rice chromosomes were used to detect nine major hybrid rice combinations and their parents in China. Out of the primers used, 21 were polymorphic and amplified 62 bands totally with a mean of 2.95 bands per primer. It could distinguish all restorer lines and most sterile lines effectively. The bands of hybrid rice were complement types of their parents, which were adapted to examine purity of hybrid rice seed. Primer RM17 was selected for SSR identification with 100 single seeds of Shanyou 63 and Liangyoupeijiu. The purity was 96.0% and 98.0%, respectively, which were approximate to their field purity (96.2% and 97.7%, respectively). It suggested that SSR technique has wide prospective in variety authentication and purity identification.
【Fund】: 国家农业科技跨越计划 (1 999- 0 1 - 1 5);; 农业部水稻学重点实验室开放项目(0 0 0 2 0 3)
【CateGory Index】: S511
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