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《Chinese Journal of Rice Science》 2003-01
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Transformation of Rice with mtlD Gene

WANG Hui zhong 1,2 , LIU Jun jun 3, LU De zhao 2, ZHAO Yan 4, YAN Mei xian 2, QIAN Qian 2, PENG Xue xian 3, CHEN Shou yi 3, HUANG Da nian 2 ( 1 Key Laboratory of Biochemistry and Molecular Biology, Hangzhou Normal College, Hangzhou 310036, China; 2China National Rice Research Institute, Hangzhou 310006, China; 3Laboratory of Plant Biotechnology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Bejing 100101, China; 4Hangzhou Institute of Commerce, Hangzhou 310035, China)  
PCR and Southern blot analysis indicated that mtlD gene had been integrated into the rice genome mediated by Agrobacterium tumefaciens LBA4404 (pBIM). mtlD gene segragated in T 1 generation and pure transgenic lines appeared in T 2 generation. The expression of the mtlD gene in transgenic plants was demonstrated by enzymatic activity assay. Under 0 75% NaCl stress, the relative electronic conductivity and membrane permeability were detected in T 3 transgenic plants of 7 different lines. Results showed that the damage to membrane structure of the transgenic plant was lower than that of their controls. Some transgenic plant lines could grow normally under 1.0% NaCl stress whereas the controls could not grow and died after 2 weeks under the same environment. Through sexual hybridization, rice plants with mtlD gene and gutD gene were got, some of them could grow normally under the stress of 1.25% NaCl.
【Fund】: 浙江省自然科学基金资助项目(3982 97;;30 2 0 61 );; 国家自然科学基金资助项目(39970 4 0 9);; 中国水稻科学发展基金资助项目 (0 0 0 32 39)
【CateGory Index】: S511
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