Ferritin Transgenic Rice Plants are Tolerant to Oxidative Stress and Magnaporthe grisea Infection
CHENG Zhi qiang 1, GUO Ze jian 1,* , XU Xiao hui 1, CAI Rong yao 2, LI De bao 1( 1Biotechnology Institute, Zhejiang University, Hangzhou 310029, China; 2Institute of Plant Protection, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; *Corresponding author, E mail:firstname.lastname@example.org)
PCR analysis on 53 ferritin transgenic plants showed that 52 T 1 transgenic plants existed a pea ferritin gene. Photosynthetic function of the transgenics with 60 μmol/L paraquat treatment was determined by measurement of maximum photochemical efficiency (F v/F m value) and chlorophyll content. Results indicated that transgenic plants exhibited a various degree of tolerance to oxidative stress. Accumulation of mRNA of Fer gene was demonstrated by Northern blot analysis. Results showed the accumulation of mRNA level was elevated in 5 of 9 plants determined. T 2 progeny of transgenic plants showed resistance to Magnaporthe grisea comparing with the non transgenic plants. The average number of lesion in transgenic plants leaves inoculated with Magnaporthe grisea was decreased significantly. These results suggested the overexpression of Fer gene in rice enhanced its resistance to oxidative damage and Magnaporthe grisea infection.
【CateGory Index】： S435.11