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《Chinese Journal of Rice Science》 2003-01
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Ferritin Transgenic Rice Plants are Tolerant to Oxidative Stress and Magnaporthe grisea Infection

CHENG Zhi qiang 1, GUO Ze jian 1,* , XU Xiao hui 1, CAI Rong yao 2, LI De bao 1( 1Biotechnology Institute, Zhejiang University, Hangzhou 310029, China; 2Institute of Plant Protection, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; *Corresponding author, E mail:zjguo@mail.hz.zj.cn)  
PCR analysis on 53 ferritin transgenic plants showed that 52 T 1 transgenic plants existed a pea ferritin gene. Photosynthetic function of the transgenics with 60 μmol/L paraquat treatment was determined by measurement of maximum photochemical efficiency (F v/F m value) and chlorophyll content. Results indicated that transgenic plants exhibited a various degree of tolerance to oxidative stress. Accumulation of mRNA of Fer gene was demonstrated by Northern blot analysis. Results showed the accumulation of mRNA level was elevated in 5 of 9 plants determined. T 2 progeny of transgenic plants showed resistance to Magnaporthe grisea comparing with the non transgenic plants. The average number of lesion in transgenic plants leaves inoculated with Magnaporthe grisea was decreased significantly. These results suggested the overexpression of Fer gene in rice enhanced its resistance to oxidative damage and Magnaporthe grisea infection.
【CateGory Index】: S435.11
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