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《Chinese Journal of Rice Science》 2013-01
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Positional Cloning and Expression Analysis of the Gene Responsible for the High-tillering Dwarf Phenotype in the indica Rice Mutant gsor23

WANG Tao1,YUAN Shou-jiang2,YIN Liang2,ZHAO Jin-feng1,WAN Jian-min1,3,LI Xue-yong1,(1 National Key Facility for Crop Gene Resources and Genetic Improvement,Institute of Crop Science,Chinese Academy of Agriculture Sciences,Beijing 100081,China;2Shandong Rice Research Institute,Jinan 250100,China;3 National Key Laboratory for Crop Genetics and Germplasm Enhancement,Jiangsu Plant Gene Engineering Research Center,Nanjing Agricultural University,Nanjing 210095,China)  
High-tillering dwarf mutant gsor23 was generated from the indica rice variety Indica9 by γ-ray radiation.Genetic analysis showed that this phenotype was controlled by one single recessive gene,which was mapped within a physical distance of 386 kb between two InDel markers C1-WT2 and C1-WT4 on the long arm of chromosome 1.There is a known gene D10 within this region and mutations in which caused high-tillering phenotype in rice.Sequence analysis of the D10 allele in gsor23 revealed that the base C at the 404th position in the coding region was deleted,causing frameshift mutation after the 134th amino acids.This mutant differed from the previously reported japonica mutants d10-1 and d10-2 in mutation site and genetic background.Therefore,gsor23 is a novel allellic mutant of D10,which encodes the carotenoid-cleaving dioxygenase 8(CCD8),a key enzyme involved in the biosynthesis of the new plant hormone strigolactones(SLs).After treatment with GR24,a synthetic analogues of SLs,gsor23 showed normal phenotype.Real-time RT-PCR analysis showed that D10 expression level was high in root,but low in leaves.Compared with the wild-type Indica9,the expression level of the SLs biosynthetic gene D10 was upregulated,while genes likely involved in the SLs signal transduction such as D3 and D14 were downregulated in the gsor23 mutant.
【Fund】: 国家转基因生物新品种培育科技重大专项(2011ZX08009-003);; 留学人员科技活动择优资助项目
【CateGory Index】: S511.21
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