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《Chinese Journal of Rice Science》 2013-01
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Function Analysis of a Rice Zinc Finger Protein Gene OsZRL by RNA Interference

YU Xiao-hong1,CHAO Li1,CHEN Chang-dong1,LIU Yong-sheng1,2,NIU Xiang-li 1,2,(1College of Life Sciences,Chongqing University,Chongqing 400044,China;2School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China)  
The function of a rice zinc-finger protein gene(OsZRL) was investigated by RNA interference.The results of semi-quantitative RT-PCR and quantitative PCR analysis indicated that OsZRL expression levels were significantly downregulated in the transgenic plants.The OsZRL-RNAi transgenic lines were characterized by bigger leaves,stronger roots and stems compared to wild type plants,indicating that the suppression of OsZRL had a positive effect on rice growth.The expression pattern and transgenic seedlings phenotypes revealed that OsZRL was involved in gibberellin or abscisic acid signaling pathway.It suggests that zinc-finger protein OsZRL might perform its function of negetive regulator in rice growth through the regulation of gibberellin and abscisic acid.
【Fund】: 国家自然科学基金资助项目(30971752 30970260);; 重庆市科技攻关计划资助项目(2010AA1019)
【CateGory Index】: S511
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