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《Chinese Journal of Rice Science》 2013-01
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Cloning of an Inorganic Pyrophosphatase Gene OsIP1 in Rice Based on Bioinformatics Analysis and its Genetic Transformation

ZHANG Ya-fang,YU Yong-qi,ZUO Shi-min,LOU Li-juan,CHEN Zong-xiang,PAN Xue-biao(Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of Education Ministry,Yangzhou University,Yangzhou 225009,China)  
Inorganic pyrophosphatase hydrolyzes inorganic pyrophosphate(PPi) to two ions of phosphate radical(Pi) in vivo.The timely degradation of PPi has been recognized as a key rate-limiting step in the process of disaccharide synthesis,especially sucrose.However,maintaining certain concentration of PPi in vascular bundle cells is required for the translocation of sucrose.Therefore,it could be the best resolution to over-express inorganic pyrophosphatase gene only in mesophyll tissue,which would feed back and accelerate photosynthesis and produce more carbohydrate.In this research,we firstly analyzed 30 inorganic pyrophosphatase genes predicted in rice by a series of bioinformatics softwares,such as protein clusters,amino acids sequence alignment,protein subcellular localization prediction and cis-elements analysis.Secondly,one gene(Os04g0687100) that was considered encoding soluble cytoplasmic inorganic pyrophosphatase,named as OsIP1,was cloned and placed under the rice mesophyll-specific promoter cyFBPase from a cytosolic fructose-1,6-bisphosphatase gene.Thirdly,we transformed this chimeric gene,cyFBPase:OsIP1,into two different source-sink type rice varieties using Agrobacterium-mediated method,and obtained a total of 48 independent positive transgenic plants.
【Fund】: 国家自然科学基金资助项目(30800067);; 中国科学院创新基金资助项目;; 江苏高校优势学科建设工程资助项目
【CateGory Index】: S511
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Chinese Journal Full-text Database 1 Hits
1 ZHANG Ya-fang,CHEN Zong-xiang,ZUO Shi-min,LOU Li-juan,YU Yong-qi,PAN Xue-biao(Key Laboratory of Crop Genetics and Physiology of Jiangsu Province,College of Agriculture,Yangzhou University/Key Laboratory of Plant Functional Genomics of the Ministry of Education,Yangzhou 225009);Effect of Over-expressing OsIP1,a Rice Inorganic Pyrophosphatase Gene,Specifically in Mesophyll Tissue of Different Source-sink Type Rice Cultivars[J];Chinese Journal of Rice Science;2013-02
Chinese Journal Full-text Database 9 Hits
1 Zhang Ning 1 Si Huaijun 1,2* Liu Na 2 Li Youzhong 2,3 Liu Haiying 2,4 Wen Gang 1,2 Wang Di 2 1 College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070; 2 Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement, Gansu Agricultural University, Lanzhou, 730070; 3 Center for Molecular Agro-biotechnology and Breeding, Xinjiang Academy of Agri- cultural and Reclamation Science, Shihezi, 832000; 4 Potato Engineering Department, Wulanchabu Vocational College, Wulanchabu, 012000;Expression in Escherichia coli and Protein Characteristic Analysis of cDNA Encoding Inorganic Pyrophosphatase of Potato[J];Molecular Plant Breeding;2008-06
2 ZHANG Ya-fang, ZUO Shi-min, WANG Li, ZHANG Ping, CHU Cheng-cai, PAN Xue-biao (Key Lab of Crop Gen Engin of Jiangsu Prov, Yangzhou Univ, Yangzhou, 225009, China; Inst of Gen and Dev Bio, Chinese Acad of Sci, Beijing, 100101, China);An excellent tissue culture indice rice cultivar[J];Journal of Yandzhou University;2005-04
3 ZHANG Yafang,CHEN Zongxiang,LOU Lijuan,ZUO Shimin,PAN Xuebiao(Key Lab of Crop Gen and Physiol of Jiangsu Prov/Key Lab of Plant Funct Genomics of MOE,Yangzhou Univ,Yangzhou 225009,China);Analysis on sourcesink relations for several rice varieties[J];Journal of Yangzhou University(Agricultural and Life Science Edition);2011-01
4 Huang Dongjie Zhang Shuzhen Feng Cuilian Guo Lihong Fan Haikuo State Key Biotechnology Laboratory for Tropical Crops,Chinese Academy of Tropical Agriculture Sciences Haikou Hainan 571101;Cloning of Inorganic Pyrophosphatase Gene and Construction of Its Plant Expression Vector[J];Chinese Journal of Tropical Crops;2007-02
5 LI Xiao yuan,ZENG Ri zhong,XIAO Xian zhou(Rubber Research Institute /Ministry of Agriculture Key Lab for Tropical Corps Physiology, CATAS,Danzhou 571737,Hainan,China);Advances on the Research of Pyrophosphatase in Plants[J];Life Science Research;2004-S2
6 Si Lizhen Chu Chengcai (Institute of Genetics and Developmental Biology CAS Beijing 100101);Manipulation of Sucrose Synthesis in Transgenic Plants[J];Progress In Biotechnology;2003-01
7 Zhao Lihong Wang Jungang Yang Benpeng Zhang Shuzhen Huang Dongjie Cai Wenwei(Key Laboratory of Tropical Crop Biotechnology,Ministry of Agriculture,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101);Genetic Transformation of Inorganic Pyrophosphatase Gene of Sugarcane[J];Biotechnology Bulletin;2009-09
9 HUANG Jian_Qiu *, WEI Zhi_Ming, AN Hai_Long, XU Shu_Ping, ZHANG Bing [WT6BZ](National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology, The Chinese Academy of Sciences, Shanghai 200032, China);High Efficiency of Genetic Transformation of Rice Using Agrobacterium Mediated Procedure[J];Acta Botanica Sinica;2000-11
Chinese Journal Full-text Database 10 Hits
1 Shui Hongxia Tang Tianze(Agricultural Science Institute of Mianyang,Mianyang,Sichuan 621023);Progress of Study on Relationship between Organs and Yield in Rape[J];Anhui Agricultural Science Bulletin;2007-16
2 Jiang Zhixun (Anhui Agricultural University, Hefei 230036);Effect of the Planting Density and Nitrogenous Fertilizer Application in Sink-limited Type in Middle Japonica Rice on Enlarged Sink and Increased Production[J];JOURNAL OF ANHUI AGRICULTURAL SCIENCES;2000-01
3 HUANG Sheng-mou (Chemical and Biological Department of Xiangfan College,Xiangfan,Hubei 441053);Effects of Sink-Source Relation on Roots activity in Hybrid Rice[J];Journal of Anhui Agricultural Sciences;2008-21
4 WANG Lan et al (Key Laboratory of Plant Molecular Breeding of Guangdong Province,College of Agriculture,South China Agricultural University,Guangzhou,Guangdong 510642);Progress in Genetic Transformation Mediated by Agrobacterium tumefacien in Rice[J];Journal of Anhui Agricultural Sciences;2009-30
5 QIU Xian-kun et al(Agricultural College of Yanbian University,Longjing,Jilin 133400);Study on Grain Filling Characters of Large Varieties of Rice[J];Journal of Anhui Agricultural Sciences;2010-04
6 LI Yi song 1,HUANG Pi sheng 2,HUANG Zhong qing 1,DING Yan feng 2 (1 Department of Agronomy,Anhui Agricultural University,Hefei 230036; 2 Key Lab of Crop Growth Regulation,Ministry of Agriculture, Nanjing Agricultural University);Study on Sourse-sink Characteristics of Two-line Indica Hybrid Rice[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;2001-04
7 SHENG Dahai, LIU Yuanying, LI Guangyu (Resources and Environmental Sciences College, Northeast Agricultural University, Harbin 150030, China);Advances and applications of research on source-sink relationship of rice[J];Journal of Northeast Agricultural University;2009-05
8 WANG Jian-wu1,2,SHEN Yu-hu1,WANG Pei1,2,WANG Hai-qing1(1 Northwest Plateau Institute of Biology,Chinese Academy of Sciences,Xining 810001,China;2 Graduate University of Chinese Academy of Sciences,Beijing 100049,China);Cloning and Characterization of A Vacuolar H~+-PPase Gene from Medicago truncatula[J];Acta Botanica Boreali-Occidentalia Sinica;2009-03
9 ZHANG Chang-wei1,2,ZHANG Shu-ning1,2,HOU Xi-lin1,2,QI Li1,2,SUN Fei-fei1,2(1 State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China;2 Key Laboratory of Southern Vegetable Crop Genetic Improvement,Ministry of Agriculture,Nanjing 210095,China);Differential Expression Analysis of Bud of Pol CMS and Its Maintainer Line of Non-heading Chinese Cabbage[J];Acta Botanica Boreali-Occidentalia Sinica;2010-08
10 GUO Yuchun, LIN Wenxiong, LIANG Yiyuan, CHEN Fangyu, LIANG Kangjing (College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Physioecological studies on dry matter production and yield formation in new plant type (NPT) rice Ⅳ. Characteristics of source and sink in NPT rice[J];Journal of Fujian Agricultural University(Natural Science);2002-04
China Proceedings of conference Full-text Database 2 Hits
1 Zhao Quanzhi Yin Chunyuan Ning Huifeng;Study on the relationship between vascular bundle structure of spike and inferior grain plumpness[A];[C];2007
2 LI Xia YAN Li-na ZHOU Yue-lan (Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China);Effect of Different Factors on Tissue Culture Power from Mature Embryo of Japonica Rice Suhuxiangjing[A];[C];2008
Chinese Journal Full-text Database 10 Hits
1 YAO Qing-rong et al(State Key Laboratory of Tropical Crop Biotechnology,Institute of Tropical Bioscience and Biotechnology,CATAS,Haikou,Hainan 571101);Current Situation and Development Prospect of Genetic Engineering in Cassava Breeding[J];Journal of Anhui Agricultural Sciences;2007-06
2 LI Guo-peng et al(Institute of Techniques for Agricultural Biology,Guangdong Ocean University,Zhanjiang,Guangdong,524088);Study on Correlation of Quality Characters and between Quality Characters and Agronomic Characters of Hybrid Rice[J];Journal of Anhui Agricultural Sciences;2007-35
3 JIAN Li et al (College of Agronomy and Biotechnology,Southwest University,Chongqing 400715);Determination and Analysis of SPAD Value and Chlorophyll Contents in Cymbidium from Guizhou[J];Journal of Anhui Agricultural Sciences;2009-35
4 WANG Juan(School of Life Sciences,Ningxia University,Yinchuan,Ningxia 750021);Optimization of Conditions Agrobacterium-mediated Transformation in Kentucky Bluegrass[J];Journal of Anhui Agricultural Sciences;2010-01
5 CHEN Dong-peng,YU Li-jie(College of Biological Science and Technology,Harbin Normal University,Harbin,Heilongjiang 150025);Establishment of Tomato Transformation Technique Via the Pollen tube Pathway Method[J];Northern Horticulture;2010-14
6 WANG Hong xia,ZHANG Zhi hua,XUAN Li chun(1.College of Horticulture, Agricultural University of Hebei,Baoding 071001,China;2.Forestry Bureau of Qian'an City, Tangshan 063000, China);Advances in research of photosynthesis in fruit trees[J];Journal of Agricultural University of Hebei;2003-S1
7 Hao Naibin Ge Qiaoying Zhang YuzhuXu Ji Zhang Guozheng Tan Kehui (Institute of Eotany, Academia sinica, Beijing)Du Weiguang Zhang Guiru Luan XiaoyanHuang Chengyun Institute of Soybean, Heilongjiang Academy of Agricultural Sciences);STUDIES ON THE PHOTOSYNTHETIC PROPERTIES OF SOYBEAN WITH PHOTOSYNTHETIC EFFICIENCY[J];Soybean Science;1989-03
8 WU Xiao-xia1,LIU Wei-ting2,LIU Qi3,LI Jing1,MA Yong4,LI Wen-bin1 (1.Agricultural College of Northeast Agricultural University,Chinese Education Ministry's and Provincial Key Laboratory of Soybean Biology,Harbin 150030;2.Plant Virus-free Nursery Stock Institute of Heilongjiang Academy of Agricultural Sciences,Harbin 150086;3.Horticulture Branch of Heilongjiang Academy of Agricultural Sciences,Harbin 150086;4.Government Agricultural Service Center of Xinxing District Changxing Township in Qitaihe,Qitaihe 154532,Heilongjiang,China);Introduction of Anti-pest Gene (cry V) to Soybean via Pollen-Tube Pathway[J];Soybean Science;2010-04
9 DUAN Wei1,LI Xinguo1,2,MENG Qingwei1*,ZHAO Shijie1 (1 College of Life Science,Shandong Agricultural University,Taian,Shandong 271018,China;2 Institute of Crop,Shandong Academy of Agricultural Sciences,Jinan 250100,China);Photoinhibiton mechanisms of plant under low temperature[J];Acta Botanica Boreali-occidentalia Sinica;2003-06
10 YIN Jun, YU Gui-rong, REN Jiang-ping, LI Lei ,SONG Li(National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou 450002,China);Transforming Anti-TrxS gene into wheat by means of pollen tube pathway and ovary injection[J];Acta Botanica Boreali-occidentalia Sinica;2004-05
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Wu Lu-guang(Hen?n-Agricultural University);PRELIMINARY RESEARCH ON FUNCTION OF PEROXIDASE IN ACER MONO SEED DORMANCY TO GERMINATION[J];Journal of Northeast Forestry University;1987-06
2 LI Guo feng,SONG Ping,CAO Xian zu,LIANG Jian sheng,ZHU Qing sen\j(Laboratory of Crop Cultivation and Physiology,Agricultural College of Yangzhou University,Yangzhou,Jiangsu 225009,China);Studies on the relationship between grain sink activity of Japonica/Indica hybrid rice and its grain filling[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;2000-02
3 Liu Haiying 1 Li Youzhong 1 Liu Na 1 Si Huaijun 1,2 Wang Di 1 1 Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement; 2 College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070;Cloning of Potato Inorganic Pyrophosphatase cDNA and Construction of Antisense Plant Expression Vectors[J];Molecular Plant Breeding;2008-01
4 Zhang Ning 1 Si Huaijun 1,2* Liu Na 2 Li Youzhong 2,3 Liu Haiying 2,4 Wen Gang 1,2 Wang Di 2 1 College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070; 2 Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement, Gansu Agricultural University, Lanzhou, 730070; 3 Center for Molecular Agro-biotechnology and Breeding, Xinjiang Academy of Agri- cultural and Reclamation Science, Shihezi, 832000; 4 Potato Engineering Department, Wulanchabu Vocational College, Wulanchabu, 012000;Expression in Escherichia coli and Protein Characteristic Analysis of cDNA Encoding Inorganic Pyrophosphatase of Potato[J];Molecular Plant Breeding;2008-06
5 WANG Xin-yan,HE Hui-ling,LIANG Guan-sheng,ZHAO Chun-xiang (College of Agriculture and Landscape Architecture,Zhongkai University of Agricultural Technology,Guangzhou,Guangdong 510225,China);Study on the dormancy of mume cultivars in southern subtropical area of China[J];Journal of Fruit Science;2007-03
6 SONG Min,LI Yuan ya,ZHANG Yun sun (Department of Biotechnology,Yunnan University,Kunming Yunnan 650091,China);Studies on Transgenic Rice(Oryza sativa L.)Plants Transformed with AGPase Gene and Its Economic Characters[J];Acta Agriculturae Boreall—sinica;2001-04
7 WANG Peng,LIAN Yong,JIN Liping(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing100081,China);The Research on the Regulation of Enzymes During Dormancy and Dormancy Releasing[J];Acta Agriculturae Boreall—sinica;2003-01
8 Qi Huaxiong et al (Food Crop Research Institute,Hubei Academy of Agricultural Scienes,Wuhan 430064,China);Application studies on the transgenic rice which resistance to brown plant hopper[J];Hubei Agricultural Sciences;2001-03
9 Tang Xiangru Guan Chunyun Yu Tieqiao (College of Plant Science and Technology,HNAU,Changsha,410128);Studies on Matter Metabolism of Yield and Quality of Different Genotypic Rice[J];JOURNAL OF HUNAN AGRICULTURAL UNIVERSITY;1999-04
10 HE Ying chun,GAO Bi da (College of Plant Science and Technology,HNAU,Changsha 410128,PRC);Construction of a New Plant Transformation Plasmid pBG1121 Containing a Tobacco Chitinase Gene and Rice Transformation[J];Journal of Hunan Agricultural University;2002-02
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