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《Chinese Journal of Rice Science》 2013-01
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Characterization and Gene Mapping of a Dwarf Mutant Zj88d in Rice

JI Xian-jun1,2,YE Sheng-hai2,ZHOU Ya2,3,XIU Fen-lian2,3,DENG Xiao-mei1,2,SHANG Hai-xuan2,3,LIU Ji-yun2,3,CHEN Ping-ping2,3,LI Xiao-hua1,2,JIN Qing-sheng2,ZHANG Xiao-ming2,(1College of Life and Environment Sciences,Hangzhou Normal University,Hangzhou 310036,China;2Institute of Crop and Nuclear Technology Utilization,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;3College of Chemistry and Life Science,Zhejiang Normal University,Jinhua 321004,China)  
The stably inherited dwarf mutant Zj88d was produced by treating the dry seeds of japonica rice variety Zhejing 88 with ethyl methylsulfonate(EMS).Genetic analysis of the dwarf mutant Zj88d revealed that this trait is controlled by one pair of recessive nuclear gene.The gene was located in the interval of 603 kb between 7-44w and RM5344 markers on the short arm of chromosome 7 by map-based cloning.The dwarf gene "OSH15" was identified by screening one hundred candidate genes on the target chromosomal segment.Sequencing results further revealed that Zj88d gene has a single-base substitution(g to a) in the last nucleotide of intron 2 of OSH15 allele.
【Fund】: 国家自然科学基金资助项目(31071207 31100142);; 浙江省科技项目(2007C12902 2010R50033);; 公益性行业(农业)科研专项(201103007)
【CateGory Index】: S511
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