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《Chinese Journal of Rice Science》 2013-01
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Effect of High Temperature on Aspartate Metabolism Enzyme Activities and Aspartate-family Amino Acids Contents at Rice Grain-filling Stage

LIANG Cheng-gang,ZHANG Qing,LI Jing,XIONG Dan,XU Guang-li,WANG Yan,LIU Quan,HUANG Peng,LI Tian(Key Laboratory of Crop Physiology,Ecology,and Cultivation in Southwest China,Ministry of Agriculture,Sichuan Agricultural University,Chengdu 611130,China)  
The effect of high temperature(HT) on aspartate aminotransferase(AAT),aspartokinase(AK) activities and the contents of Asp-family amino acids and protein components in grains were measured at rice grain filling stage with japonica rice variety Koshihikari and indica rice variety IR72 as materials.The results indicated that the activities of AAT and AK and the relative contents of Asp,Lys,Thr,Met and Ile in grains significantly increased under HT,and AAT and AK activities were significantly positively correlated with Asp-family amino acids contents,suggesting that AAT and AK in rice grains may play an important role in the increase of Asp-family amino acids contents in grains under HT.Additionally,the results indicated that HT during grain filling contributed to the increase of relative contents of protein components and Asp-family amino acids contents,the accumulation of glutelin and Asp-family amino acids,and the improvement of the ratio of part of Asp-family amino acids contents in crude protein.However,the contents of protein components and part of Asp-family amino acids in spikelet decreased under HT during rice grain filling.
【Fund】: 四川省国际科技合作与交流研究计划资助项目(2010HH0015);; 四川省科技计划资助项目(2009ZR0109);; 四川农业大学大学生科研兴趣培养计划资助项目
【CateGory Index】: S511
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